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Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea
https://repository.naro.go.jp/records/3438
https://repository.naro.go.jp/records/3438ae6c7ac1-01cc-4c3e-86c1-fb20da195219
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-02 | |||||
タイトル | ||||||
タイトル | Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lasso peptide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heterologous production | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sphingomonas subterranea | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Brevundimonas diminuta | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NMR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Lasso peptide | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Heterologous production | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sphingomonas subterranea | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Brevundimonas diminuta | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | NMR | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
逸見, 光
× 逸見, 光× 中川, 博之 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes. | |||||
書誌情報 |
Journal of Industrial Microbiology & Biotechnology en : Journal of Industrial Microbiology & Biotechnology 巻 45, 号 11, p. 983-992, 発行日 2018-09-06 |
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出版者 | ||||||
出版者 | Springer Nature | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1476-5535 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10295-018-2077-6 | |||||
権利 | ||||||
権利情報 | The Author(s) | |||||
情報源 | ||||||
関連名称 | SC30201903070022 | |||||
情報源 | ||||||
関連名称 | NARO成果DBa | |||||
情報源 | ||||||
関連名称 | This is a post-peer-review, pre-copyedit version of an article published in [Journal of Industrial Microbiology & Biotechnology]. | |||||
情報源 | ||||||
関連名称 | The final authenticated version is available online at: https://doi.org/10.1007/s10295-018-2077-6 | |||||
情報源 | ||||||
関連名称 | author manuscript | |||||
情報源 | ||||||
関連名称 | このアーカイブは著者版です。出版社版ではありません。引用の際には出版社版をご利用ください。 | |||||
情報源 | ||||||
関連名称 | This is not the published version. Please cite only the published version. | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s10295-018-2077-6 | |||||
関連名称 | Journal of Industrial Microbiology & Biotechnology | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |