{"_buckets": {"deposit": "83e0be92-cd68-4785-b414-af943c9c8362"}, "_deposit": {"created_by": 11, "id": "3438", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "3438"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00003438", "sets": ["45", "50", "62"]}, "author_link": ["756", "352"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-09-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "992", "bibliographicPageStart": "983", "bibliographicVolumeNumber": "45", "bibliographic_titles": [{"bibliographic_title": "Journal of Industrial Microbiology \u0026 Biotechnology"}, {"bibliographic_title": "Journal of Industrial Microbiology \u0026 Biotechnology", "bibliographic_titleLang": "en"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer Nature"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1007/s10295-018-2077-6", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "SC30201903070022"}]}, {"subitem_relation_name": [{"subitem_relation_name_text": "NARO成果DBa"}]}, {"subitem_relation_name": [{"subitem_relation_name_text": "This is a post-peer-review, pre-copyedit version of an article published in [Journal of Industrial Microbiology \u0026 Biotechnology]. "}]}, {"subitem_relation_name": [{"subitem_relation_name_text": "The final authenticated version is available online at: https://doi.org/10.1007/s10295-018-2077-6"}]}, {"subitem_relation_name": [{"subitem_relation_name_text": "author manuscript "}]}, {"subitem_relation_name": [{"subitem_relation_name_text": "このアーカイブは著者版です。出版社版ではありません。引用の際には出版社版をご利用ください。"}]}, {"subitem_relation_name": [{"subitem_relation_name_text": "This is not the published version. Please cite only the published version. "}]}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Journal of Industrial Microbiology \u0026 Biotechnology"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s10295-018-2077-6", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "The Author(s) "}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1476-5535", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "逸見, 光"}, {"creatorName": "ヘンミ, ヒカル", "creatorNameLang": "ja-Kana"}, {"creatorName": "HEMMI, Hikaru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "756", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70353993", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70353993"}, {"nameIdentifier": "read0004154", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/read0004154"}]}, {"creatorNames": [{"creatorName": "中川, 博之"}, {"creatorName": "ナカガワ, ヒロユキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "NAKAGAWA, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "352", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "30308192", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=30308192"}, {"nameIdentifier": "NaroFri", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/NaroFri"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-11-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "SC30201903070022_PostPrint-a.pdf", "filesize": [{"value": "586.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 586000.0, "url": {"label": "SC30201903070022_PostPrint", "url": "https://repository.naro.go.jp/record/3438/files/SC30201903070022_PostPrint-a.pdf"}, "version_id": "214e040e-11dd-47bf-a75e-688032d5e268"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Lasso peptide", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Heterologous production", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sphingomonas subterranea", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brevundimonas diminuta", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NMR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lasso peptide", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Heterologous production", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sphingomonas subterranea", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brevundimonas diminuta", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NMR", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea"}, {"subitem_title": "Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "11", "path": ["45", "50", "62"], "permalink_uri": "https://repository.naro.go.jp/records/3438", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-06-02"}, "publish_date": "2020-06-02", "publish_status": "0", "recid": "3438", "relation": {}, "relation_version_is_last": true, "title": ["Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea"], "weko_shared_id": -1}