{"created":"2023-05-15T13:39:15.648676+00:00","id":3438,"links":{},"metadata":{"_buckets":{"deposit":"83e0be92-cd68-4785-b414-af943c9c8362"},"_deposit":{"created_by":11,"id":"3438","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3438"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003438","sets":["2:581:62","87:551:680:50","87:627:45"]},"author_link":["756","352"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-09-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"992","bibliographicPageStart":"983","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Journal of Industrial Microbiology & Biotechnology"},{"bibliographic_title":"Journal of Industrial Microbiology & Biotechnology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10295-018-2077-6","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC30201903070022"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This is a post-peer-review, pre-copyedit version of an article published in [Journal of Industrial Microbiology & Biotechnology]. "}]},{"subitem_relation_name":[{"subitem_relation_name_text":"The final authenticated version is available online at: https://doi.org/10.1007/s10295-018-2077-6"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"author manuscript "}]},{"subitem_relation_name":[{"subitem_relation_name_text":"このアーカイブは著者版です。出版社版ではありません。引用の際には出版社版をご利用ください。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This is not the published version. Please cite only the published version. "}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Industrial Microbiology & Biotechnology"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s10295-018-2077-6","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The Author(s) "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1476-5535","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"逸見, 光"},{"creatorName":"ヘンミ, ヒカル","creatorNameLang":"ja-Kana"},{"creatorName":"HEMMI, Hikaru","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中川, 博之"},{"creatorName":"ナカガワ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAGAWA, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-11-01"}],"displaytype":"detail","filename":"SC30201903070022_PostPrint-a.pdf","filesize":[{"value":"586.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC30201903070022_PostPrint","url":"https://repository.naro.go.jp/record/3438/files/SC30201903070022_PostPrint-a.pdf"},"version_id":"214e040e-11dd-47bf-a75e-688032d5e268"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Lasso peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"Heterologous production","subitem_subject_scheme":"Other"},{"subitem_subject":"Sphingomonas subterranea","subitem_subject_scheme":"Other"},{"subitem_subject":"Brevundimonas diminuta","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_scheme":"Other"},{"subitem_subject":"Lasso peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Heterologous production","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sphingomonas subterranea","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Brevundimonas diminuta","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea"},{"subitem_title":"Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["45","50","62"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-06-02"},"publish_date":"2020-06-02","publish_status":"0","recid":"3438","relation_version_is_last":true,"title":["Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-02-22T01:48:11.407848+00:00"}