@article{oai:repository.naro.go.jp:00003438,
 author = {逸見, 光 and HEMMI, Hikaru and 中川, 博之 and NAKAGAWA, Hiroyuki},
 issue = {11},
 journal = {Journal of Industrial Microbiology & Biotechnology, Journal of Industrial Microbiology & Biotechnology},
 month = {Sep},
 note = {A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.},
 pages = {983--992},
 title = {Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea},
 volume = {45},
 year = {2018},
 yomi = {ヘンミ, ヒカル and ナカガワ, ヒロユキ}
}