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Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa
https://repository.naro.go.jp/records/2631
https://repository.naro.go.jp/records/2631d7319f36-a9be-4dd8-8028-6bb7eb7aec8d
| 名前 / ファイル | ライセンス | アクション |
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SC40201804180008_PostPrint (349.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2019-09-27 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa | |||||||||||
| タイトル | ||||||||||||
| タイトル | Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | epigenetics | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | fusarium wilt | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | gene expression | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | methylome | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | transposable elements | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | epigenetics | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | fusarium wilt | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | gene expression | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | methylome | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | transposable elements | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
板橋, 悦子
× 板橋, 悦子
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | DNA methylation is an epigenetic gene regulatory mechanism that plays an essential role in gene expression, transposon silencing, genome imprinting, and plant development. We investigated the influence of DNA methylation on gene expression in Brassica rapa, to understand if there are epigenetic differences between inbred lines. Genome-wide DNA methylation was analyzed by Methylated DNA Immunoprecipitation sequencing (MeDIP-seq) of 14-day-old first and second leaves from two inbred lines of Chinese cabbage that are susceptible or resistant to Fusarium yellows. Model-based analysis for ChIP-seq (MACS) identified DNA methylation peaks in genic regions including 2 kb upstream, exon, intron, and 2 kb downstream regions. More than 65 % of genes showed similar patterns of DNA methylation in the genic regions in the two inbred lines. DNA methylation states of the two inbred lines were compared to their transcriptome. Genes having DNA methylation in the intron and the 200 bp upstream and downstream regions were associated with a lower expression level in both lines. A small number of genes showed a negative correlation between difference of DNA methylation levels and difference of transcriptional levels between the two inbred lines, suggesting that DNA methylation in these genes result in transcriptional suppression. | |||||||||||
| 書誌情報 |
Crop & Pesture Science en : Crop & Pesture Science 巻 69, 号 1, p. 107-120, 発行日 2018-01-04 |
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| 出版者 | ||||||||||||
| 出版者 | CSIRO Publishing | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1836-5795 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1071/CP17394 | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | SC40201804180008 | |||||||||||
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| 関連名称 | NARO成果DBa | |||||||||||
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| 関連名称 | author manuscript | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | この論文は出版社版ではありません。引用の際には出版社版をご利用ください。 | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | This is not the published version. Please cite only the published version. | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://www.publish.csiro.au/cp/CP17394 | |||||||||||
| 関連名称 | Crop & Pesture Science | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
