{"created":"2023-05-15T13:38:37.864588+00:00","id":2631,"links":{},"metadata":{"_buckets":{"deposit":"74b7527b-6320-404e-81a7-7b0b69c5fe36"},"_deposit":{"created_by":11,"id":"2631","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2631"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002631","sets":["2:581:101","87:591:41"]},"author_link":["937"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"120","bibliographicPageStart":"107","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"Crop & Pesture Science"},{"bibliographic_title":"Crop & Pesture Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA methylation is an epigenetic gene regulatory mechanism that plays an essential role in gene expression, transposon silencing, genome imprinting, and plant development. We investigated the influence of DNA methylation on gene expression in Brassica rapa, to understand if there are epigenetic differences between inbred lines.  Genome-wide DNA methylation was analyzed by Methylated DNA Immunoprecipitation sequencing (MeDIP-seq) of 14-day-old first and second leaves from two inbred lines of Chinese cabbage that are susceptible or resistant to Fusarium yellows. Model-based analysis for ChIP-seq (MACS) identified DNA methylation peaks in genic regions including 2 kb upstream, exon, intron, and 2 kb downstream regions. More than 65 % of genes showed similar patterns of DNA methylation in the genic regions in the two inbred lines. DNA methylation states of the two inbred lines were compared to their transcriptome. Genes having DNA methylation in the intron and the 200 bp upstream and downstream regions were associated with a lower expression level in both lines. A small number of genes showed a negative correlation between difference of DNA methylation levels and difference of transcriptional levels between the two inbred lines, suggesting that DNA methylation in these genes result in transcriptional suppression.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"CSIRO Publishing"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1071/CP17394","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC40201804180008"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"author manuscript "}]},{"subitem_relation_name":[{"subitem_relation_name_text":"この論文は出版社版ではありません。引用の際には出版社版をご利用ください。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This is not the published version. Please cite only the published version. "}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Crop & Pesture Science"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.publish.csiro.au/cp/CP17394","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1836-5795","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"板橋, 悦子"},{"creatorName":"イタバシ, エツコ","creatorNameLang":"ja-Kana"},{"creatorName":"ITABASHI, Etsuko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-01"}],"displaytype":"detail","filename":"NARO-SC40201804180008_PostPrint.pdf","filesize":[{"value":"349.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC40201804180008_PostPrint","url":"https://repository.naro.go.jp/record/2631/files/NARO-SC40201804180008_PostPrint.pdf"},"version_id":"5f626e75-81af-4b54-9db4-64ce6cdeab78"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"epigenetics","subitem_subject_scheme":"Other"},{"subitem_subject":"fusarium wilt","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_scheme":"Other"},{"subitem_subject":"methylome","subitem_subject_scheme":"Other"},{"subitem_subject":"transposable elements","subitem_subject_scheme":"Other"},{"subitem_subject":"epigenetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fusarium wilt","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"methylome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transposable elements","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa"},{"subitem_title":"Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["41","101"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-09-27"},"publish_date":"2019-09-27","publish_status":"0","recid":"2631","relation_version_is_last":true,"title":["Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T15:58:11.357144+00:00"}