@article{oai:repository.naro.go.jp:00002631,
 author = {板橋, 悦子 and ITABASHI, Etsuko},
 issue = {1},
 journal = {Crop & Pesture Science, Crop & Pesture Science},
 month = {Jan},
 note = {DNA methylation is an epigenetic gene regulatory mechanism that plays an essential role in gene expression, transposon silencing, genome imprinting, and plant development. We investigated the influence of DNA methylation on gene expression in Brassica rapa, to understand if there are epigenetic differences between inbred lines.  Genome-wide DNA methylation was analyzed by Methylated DNA Immunoprecipitation sequencing (MeDIP-seq) of 14-day-old first and second leaves from two inbred lines of Chinese cabbage that are susceptible or resistant to Fusarium yellows. Model-based analysis for ChIP-seq (MACS) identified DNA methylation peaks in genic regions including 2 kb upstream, exon, intron, and 2 kb downstream regions. More than 65 % of genes showed similar patterns of DNA methylation in the genic regions in the two inbred lines. DNA methylation states of the two inbred lines were compared to their transcriptome. Genes having DNA methylation in the intron and the 200 bp upstream and downstream regions were associated with a lower expression level in both lines. A small number of genes showed a negative correlation between difference of DNA methylation levels and difference of transcriptional levels between the two inbred lines, suggesting that DNA methylation in these genes result in transcriptional suppression.},
 pages = {107--120},
 title = {Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa},
 volume = {69},
 year = {2018},
 yomi = {イタバシ, エツコ}
}