| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-12-11 |
| タイトル |
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タイトル |
Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism |
| タイトル |
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タイトル |
Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
野田, 尚信
能岡, 智
岸本, 早苗
中山, 真義
道園, 美弦
間, 竜太郎
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Various colored cultivars of ornamental flowers have been bred by hybridization and mutation breeding; however, the generation of blue flowers for major cut flower plants, such as roses, chrysanthemums, and carnations, has not been achieved by conventional breeding or genetic engineering. Most blue-hued flowers contain delphinidin-based anthocyanins; therefore, delphinidin-producing carnation, rose, and chrysanthemum flowers have been generated by overexpression of the gene encoding flavonoid 3′,5′-hydroxylase (F3′5′H), the key enzyme for delphinidin biosynthesis. Even so, the flowers are purple/violet rather than blue. To generate true blue flowers, blue pigments, such as polyacylated anthocyanins and metal complexes, must be introduced by metabolic engineering; however, introducing and controlling multiple transgenes in plants are complicated processes. We succeeded in generating blue chrysanthemum flowers by introduction of butterfly pea UDP (uridine diphosphate)–glucose:anthocyanin 3′,5′-O-glucosyltransferase gene, in addition to the expression of the Canterbury bells F3′5′H. Newly synthesized 3′,5′-diglucosylated delphinidin-based anthocyanins exhibited a violet color under the weakly acidic pH conditions of flower petal juice and showed a blue color only through intermolecular association, termed “copigmentation,” with flavone glucosides in planta. Thus, we achieved the development of blue color by a two-step modification of the anthocyanin structure. This simple method is a promising approach to generate blue flowers in various ornamental plants by metabolic engineering. |
| 書誌情報 |
Science Advances
en : Science Advances
巻 3,
号 7,
p. e1602785,
発行日 2017-07-26
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| 出版者 |
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出版者 |
American Association for the Advancement of Science (AAAS) |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2375-2548 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1126/sciadv.1602785 |
| 情報源 |
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関連名称 |
SC30201804130030 |
| 情報源 |
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関連名称 |
NARO成果DBa |
| 情報源 |
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関連名称 |
OAへのリンク https://doi.org/10.1126/sciadv.1602785 |
| 関連サイト |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1126/sciadv.1602785 |
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関連名称 |
Science Advances |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
https://www.naro.go.jp/publicity_report/press/laboratory/nivfs/076531.html |
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関連名称 |
プレスリリース (研究成果) 「青いキク」が誕生 |
