{"created":"2023-05-15T13:39:09.073652+00:00","id":3277,"links":{},"metadata":{"_buckets":{"deposit":"2999f5a5-528d-4b1b-9dc2-64a97038bb9e"},"_deposit":{"created_by":3,"id":"3277","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"3277"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003277","sets":["2:581:120","87:591:41"]},"author_link":["1497","1922","1688","1506","630","1715"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-07-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"e1602785","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Science Advances"},{"bibliographic_title":"Science Advances","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Various colored cultivars of ornamental flowers have been bred by hybridization and mutation breeding; however, the generation of blue flowers for major cut flower plants, such as roses, chrysanthemums, and carnations, has not been achieved by conventional breeding or genetic engineering. Most blue-hued flowers contain delphinidin-based anthocyanins; therefore, delphinidin-producing carnation, rose, and chrysanthemum flowers have been generated by overexpression of the gene encoding flavonoid 3′,5′-hydroxylase (F3′5′H), the key enzyme for delphinidin biosynthesis. Even so, the flowers are purple/violet rather than blue. To generate true blue flowers, blue pigments, such as polyacylated anthocyanins and metal complexes, must be introduced by metabolic engineering; however, introducing and controlling multiple transgenes in plants are complicated processes. We succeeded in generating blue chrysanthemum flowers by introduction of butterfly pea UDP (uridine diphosphate)–glucose:anthocyanin 3′,5′-O-glucosyltransferase gene, in addition to the expression of the Canterbury bells F3′5′H. Newly synthesized 3′,5′-diglucosylated delphinidin-based anthocyanins exhibited a violet color under the weakly acidic pH conditions of flower petal juice and showed a blue color only through intermolecular association, termed “copigmentation,” with flavone glucosides in planta. Thus, we achieved the development of blue color by a two-step modification of the anthocyanin structure. This simple method is a promising approach to generate blue flowers in various ornamental plants by metabolic engineering.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Association for the Advancement of Science (AAAS)"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1126/sciadv.1602785","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC30201804130030"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"OAへのリンク　https://doi.org/10.1126/sciadv.1602785"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Science Advances"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1126/sciadv.1602785","subitem_relation_type_select":"DOI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"プレスリリース (研究成果) 「青いキク」が誕生"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.naro.go.jp/publicity_report/press/laboratory/nivfs/076531.html","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2375-2548","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野田, 尚信"},{"creatorName":"ノダ, ナオノブ","creatorNameLang":"ja-Kana"},{"creatorName":"NODA, Naonobu","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"能岡, 智"},{"creatorName":"ヨシオカ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"YOSHIOKA, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"岸本, 早苗"},{"creatorName":"キシモト, サナエ","creatorNameLang":"ja-Kana"},{"creatorName":"KISHIMOTO, Sanae","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中山, 真義"},{"creatorName":"ナカヤマ, マサヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAYAMA, Masayoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"DOZONO, Mitsuru","creatorNameLang":"en"},{"creatorName":"DOUZONO, Mitsuru","creatorNameLang":"en"},{"creatorName":"道園, 美弦","creatorNameLang":"ja"},{"creatorName":"ドウゾノ, ミツル","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"間, 竜太郎"},{"creatorName":"アイダ, リュウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"AIDA, Ryutaro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism"},{"subitem_title":"Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"3","path":["41","120"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-11"},"publish_date":"2019-12-11","publish_status":"0","recid":"3277","relation_version_is_last":true,"title":["Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-20T06:17:08.062827+00:00"}