{"_buckets": {"deposit": "bb1f41e1-f5a4-48b5-8a45-c3bf6f4b7373"}, "_deposit": {"created_by": 11, "id": "222", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "222"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00000222", "sets": ["45", "63"]}, "author_link": ["2700", "2688", "2695", "2699", "2697", "2691", "2693", "2689", "2686", "2702", "2690", "1663", "1037", "2701", "2698", "2694", "2687", "2692"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "2207", "bibliographicPageStart": "2198", "bibliographicVolumeNumber": "80", "bibliographic_titles": [{"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry"}, {"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)"}, {"subitem_publisher": "Informa UK Limited, trading as Taylor \u0026 Francis Group"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1080/09168451.2016.1206810", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "SC10201706260015"}]}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Bioscience, Biotechnology, and Biochemistry"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1080/09168451.2016.1206810", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2016 The Author(s)."}, {"subitem_rights": "Published by Informa UK Limited, trading as Taylor \u0026 Francis Group"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-8451", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "SATO, Rie", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1663", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "10399371", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=10399371"}, {"nameIdentifier": "read0078418", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/read0078418"}, {"nameIdentifier": "0000-0002-0139-1766", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "http://orcid.org/0000-0002-0139-1766"}]}, {"creatorNames": [{"creatorName": "TESHIMA, Reiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2686", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KITTA, Kazumi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1037", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60353926", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60353926"}, {"nameIdentifier": "FAAU", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/FAAU"}, {"nameIdentifier": "0000-0002-3691-271X", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "http://orcid.org/0000-0002-3691-271X"}]}, {"creatorNames": [{"creatorName": "Lang, Gang-Hua", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2687", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kathleen, Schegg", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2688", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kenneth, Blumenthal", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2689", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Leslie, Hicks", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2701", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bénédicte, Labory-Carcenac", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2690", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "David, Rouquié", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2691", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Rod_A., Herman", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2692", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Corinne, Herouet-Guicheney", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2693", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Gregory_S., Ladics", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2702", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Scott, McClain", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2694", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lars_K., Poulsen", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2695", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Laura, Privalle", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2700", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jason_M., Ward", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2697", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nancy, Doerrer", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2698", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jean-Baptiste, Rascle", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2699", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-03-02"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf", "url": "https://repository.naro.go.jp/record/222/files/SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf"}, "version_id": "60de66a7-60a5-4566-a288-5b422ef198fc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "rice grain", "subitem_subject_scheme": "Other"}, {"subitem_subject": "allergen", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proteomics", "subitem_subject_scheme": "Other"}, {"subitem_subject": "multiplex immunodetection", "subitem_subject_scheme": "Other"}, {"subitem_subject": "two-dimensional difference gel electrophoresis (2D-DIGE)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rice grain", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "allergen", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proteomics", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "multiplex immunodetection", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "two-dimensional difference gel electrophoresis (2D-DIGE)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens"}, {"subitem_title": "Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "11", "path": ["45", "63"], "permalink_uri": "https://repository.naro.go.jp/records/222", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-03-02"}, "publish_date": "2018-03-02", "publish_status": "0", "recid": "222", "relation": {}, "relation_version_is_last": true, "title": ["Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens"], "weko_shared_id": -1}