WEKO3
アイテム
Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens
https://repository.naro.go.jp/records/222
https://repository.naro.go.jp/records/222ec24c008-29e8-4399-895b-91cb4dd6ce16
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2018-03-02 | |||||||
| タイトル | ||||||||
| タイトル | Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens | |||||||
| タイトル | ||||||||
| タイトル | Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | rice grain | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | allergen | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | proteomics | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | multiplex immunodetection | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | two-dimensional difference gel electrophoresis (2D-DIGE) | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | rice grain | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | allergen | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | proteomics | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | multiplex immunodetection | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | two-dimensional difference gel electrophoresis (2D-DIGE) | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 著者 |
SATO, Rie
× SATO, Rie
WEKO
1663
× TESHIMA, Reiko× KITTA, Kazumi× Lang, Gang-Hua× Kathleen, Schegg× Kenneth, Blumenthal× Leslie, Hicks× Bénédicte, Labory-Carcenac× David, Rouquié× Rod_A., Herman× Corinne, Herouet-Guicheney× Gregory_S., Ladics× Scott, McClain× Lars_K., Poulsen× Laura, Privalle× Jason_M., Ward× Nancy, Doerrer× Jean-Baptiste, Rascle |
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens. | |||||||
| 書誌情報 |
Bioscience, Biotechnology, and Biochemistry en : Bioscience, Biotechnology, and Biochemistry 巻 80, 号 11, p. 2198-2207, 発行日 2016-07 |
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| 出版者 | ||||||||
| 出版者 | The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) | |||||||
| 出版者 | ||||||||
| 出版者 | Informa UK Limited, trading as Taylor & Francis Group | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0916-8451 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1080/09168451.2016.1206810 | |||||||
| 権利 | ||||||||
| 権利情報 | © 2016 The Author(s). | |||||||
| 権利 | ||||||||
| 権利情報 | Published by Informa UK Limited, trading as Taylor & Francis Group | |||||||
| 情報源 | ||||||||
| 関連名称 | SC10201706260015 | |||||||
| 関連サイト | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1080/09168451.2016.1206810 | |||||||
| 関連名称 | Bioscience, Biotechnology, and Biochemistry | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
