{"created":"2023-05-15T13:36:43.550985+00:00","id":222,"links":{},"metadata":{"_buckets":{"deposit":"bb1f41e1-f5a4-48b5-8a45-c3bf6f4b7373"},"_deposit":{"created_by":11,"id":"222","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"222"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000222","sets":["2:581:63","87:627:45"]},"author_link":["2700","2688","2695","2699","2697","2691","2693","2689","2686","2702","2690","1663","1037","2701","2698","2694","2687","2692"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2207","bibliographicPageStart":"2198","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry"},{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)"},{"subitem_publisher":"Informa UK Limited, trading as Taylor & Francis Group"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/09168451.2016.1206810","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC10201706260015"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Bioscience, Biotechnology, and Biochemistry"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1080/09168451.2016.1206810","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016 The Author(s)."},{"subitem_rights":"Published by Informa UK Limited, trading as Taylor & Francis Group"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SATO, Rie","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"TESHIMA, Reiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KITTA, Kazumi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Lang, Gang-Hua","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kathleen, Schegg","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kenneth, Blumenthal","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Leslie, Hicks","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Bénédicte, Labory-Carcenac","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"David, Rouquié","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rod_A., Herman","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Corinne, Herouet-Guicheney","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gregory_S., Ladics","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Scott, McClain","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lars_K., Poulsen","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Laura, Privalle","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jason_M., Ward","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nancy, Doerrer","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jean-Baptiste, Rascle","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-02"}],"displaytype":"detail","filename":"SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf","url":"https://repository.naro.go.jp/record/222/files/SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf"},"version_id":"60de66a7-60a5-4566-a288-5b422ef198fc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"rice grain","subitem_subject_scheme":"Other"},{"subitem_subject":"allergen","subitem_subject_scheme":"Other"},{"subitem_subject":"proteomics","subitem_subject_scheme":"Other"},{"subitem_subject":"multiplex immunodetection","subitem_subject_scheme":"Other"},{"subitem_subject":"two-dimensional difference gel electrophoresis (2D-DIGE)","subitem_subject_scheme":"Other"},{"subitem_subject":"rice grain","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"allergen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proteomics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"multiplex immunodetection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"two-dimensional difference gel electrophoresis (2D-DIGE)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens"},{"subitem_title":"Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["45","63"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-02"},"publish_date":"2018-03-02","publish_status":"0","recid":"222","relation_version_is_last":true,"title":["Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T23:15:33.347359+00:00"}