@article{oai:repository.naro.go.jp:00000222,
 author = {SATO, Rie and TESHIMA, Reiko and KITTA, Kazumi and Lang, Gang-Hua and Kathleen, Schegg and Kenneth, Blumenthal and Leslie, Hicks and Bénédicte, Labory-Carcenac and David, Rouquié and Rod_A., Herman and Corinne, Herouet-Guicheney and Gregory_S., Ladics and Scott, McClain and Lars_K., Poulsen and Laura, Privalle and Jason_M., Ward and Nancy, Doerrer and Jean-Baptiste, Rascle},
 issue = {11},
 journal = {Bioscience, Biotechnology, and Biochemistry, Bioscience, Biotechnology, and Biochemistry},
 month = {Jul},
 note = {In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.},
 pages = {2198--2207},
 title = {Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens},
 volume = {80},
 year = {2016}
}