| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-03-02 |
| タイトル |
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|
タイトル |
Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens |
| タイトル |
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|
タイトル |
Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
rice grain |
| キーワード |
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主題Scheme |
Other |
|
主題 |
allergen |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
proteomics |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
multiplex immunodetection |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
two-dimensional difference gel electrophoresis (2D-DIGE) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
rice grain |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
allergen |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
proteomics |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
multiplex immunodetection |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
two-dimensional difference gel electrophoresis (2D-DIGE) |
| 資源タイプ |
|
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
SATO, Rie
TESHIMA, Reiko
KITTA, Kazumi
Lang, Gang-Hua
Kathleen, Schegg
Kenneth, Blumenthal
Leslie, Hicks
Bénédicte, Labory-Carcenac
David, Rouquié
Rod_A., Herman
Corinne, Herouet-Guicheney
Gregory_S., Ladics
Scott, McClain
Lars_K., Poulsen
Laura, Privalle
Jason_M., Ward
Nancy, Doerrer
Jean-Baptiste, Rascle
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens. |
| 書誌情報 |
Bioscience, Biotechnology, and Biochemistry
en : Bioscience, Biotechnology, and Biochemistry
巻 80,
号 11,
p. 2198-2207,
発行日 2016-07
|
| 出版者 |
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出版者 |
The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) |
| 出版者 |
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出版者 |
Informa UK Limited, trading as Taylor & Francis Group |
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-8451 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1080/09168451.2016.1206810 |
| 権利 |
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|
権利情報 |
© 2016 The Author(s). |
| 権利 |
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|
権利情報 |
Published by Informa UK Limited, trading as Taylor & Francis Group |
| 情報源 |
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関連名称 |
SC10201706260015 |
| 関連サイト |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1080/09168451.2016.1206810 |
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|
関連名称 |
Bioscience, Biotechnology, and Biochemistry |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
SC10201706260015_CCBYNCND_BBB80(11)p2198.pdf (1.1 MB)
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