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Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method
https://doi.org/10.24514/00006681
https://doi.org/10.24514/0000668164c65ebc-8ada-49f8-88e0-6a790d4499a0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10.24514-00006681_DOI_moved (279.3 kB)
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| Item type | 紀要一般記事 / Departmental Bulletin Article(1) | |||||||||||
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| 公開日 | 2021-12-17 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method | |||||||||||
| タイトル | ||||||||||||
| タイトル | Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | β-conglycinin | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | soybean | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Glycine max (L.) Merr. | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | liquid chromatography/tandem mass spectrometry (LC/MS/MS) | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | stable isotope-labelled peptide | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | β-conglycinin | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | soybean | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Glycine max (L.) Merr. | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | liquid chromatography/tandem mass spectrometry (LC/MS/MS) | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | stable isotope-labelled peptide | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00006681 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
一法師, 克成
× 一法師, 克成
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | β-Conglycinin, a major protein in soybeans (Glycine max (L.) Merr.), improves human lipid metabolism and influences the processing properties of soybeans. Recently, an analytical method based on the absolute quantification (AQUA) strategy for β-conglycinin was developed by us. In this study, the performance of an assay combining trichloroacetic acid (TCA)/acetone protein extraction and the AQUA method with cryogenic milling in dry ice was evaluated, which is used for measuring pesticide residues in foods to prepare homogeneous comminuted samples. The homogeneity of soybean powder obtained by cryogenic milling and the linearity and precision of the analytical assay were satisfactory; however, its recovery was not high. Guanidinium-mediated denaturation and trypsin digestion of β-conglycinin in the AQUA method, following the TCA/acetone extraction, were suggested to have a substantially higher negative effect on recovery than the TCA/acetone method. Overall, as this assay is based on AQUA strategy using stable isotope-labelled peptides as internal standards, and its performance except for recovery was satisfactory, it is thought to be available for the analysis of β-conglycinin in cases where a high trueness of measurement is not needed (e.g. comparative semi-quantification of β-conglycinin). |
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| 書誌情報 |
農研機構研究報告 en : Journal of the NARO Research and Development 巻 9, p. 51-56, 発行日 2021-11-30 |
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| 出版者 | ||||||||||||
| 出版者 | 国立研究開発法人 農業・食品産業技術総合研究機構 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | National Agriculture and Food Research Organization, Japan (NARO) | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 2434-9909 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.34503/naroj.2021.9_51 | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | 本文はJ-Stageよりご利用いただけます。 | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | This article full-text PDF is available at J-Stage. | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.34503/naroj.2021.9_51 | |||||||||||
| 関連名称 | https://doi.org/10.34503/naroj.2021.9_51 | |||||||||||
| 他の資源との関係 | ||||||||||||
| 関連名称 | 2022.2 J-Stageへ移管しました | |||||||||||
| 他の資源との関係 | ||||||||||||
| 関連名称 | We changed the journal platform to J-Stage. 2022.2 - | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
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Cite as
IPPOUSHI, Katsunari, 2021, Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method: National Agriculture and Food Research Organization, Japan (NARO), 51–56 p.
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