{"created":"2023-05-15T13:41:38.296168+00:00","id":6826,"links":{},"metadata":{"_buckets":{"deposit":"23863f1f-39ad-4a44-932d-edf12a981e73"},"_deposit":{"created_by":11,"id":"6826","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"6826"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00006826","sets":["1706970004180:1:420:578"]},"author_link":["916"],"item_10_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"56","bibliographicPageStart":"51","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"農研機構研究報告"},{"bibliographic_title":"Journal of the NARO Research and Development","bibliographic_titleLang":"en"}]}]},"item_10_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" β-Conglycinin, a major protein in soybeans (Glycine max (L.) Merr.), improves human lipid metabolism and influences the processing properties of soybeans. Recently, an analytical method based on the absolute quantification (AQUA) strategy for β-conglycinin was developed by us. In this study, the performance of an assay combining trichloroacetic acid (TCA)/acetone protein extraction and the AQUA method with cryogenic milling in dry ice was evaluated, which is used for measuring pesticide residues in foods to prepare homogeneous comminuted samples. The homogeneity of soybean powder obtained by cryogenic milling and\nthe linearity and precision of the analytical assay were satisfactory; however, its recovery was not high. Guanidinium-mediated denaturation and trypsin digestion of β-conglycinin in the AQUA method, following the TCA/acetone extraction, were suggested to have a substantially higher negative effect on recovery than the TCA/acetone method. Overall, as this assay is based on AQUA strategy using stable isotope-labelled peptides as internal standards, and its performance except for recovery was satisfactory, it is thought to be available for the analysis of β-conglycinin in cases where a high trueness of measurement is not needed (e.g. comparative semi-quantification of β-conglycinin).","subitem_description_type":"Abstract"}]},"item_10_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00006681","subitem_identifier_reg_type":"JaLC"}]},"item_10_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構"},{"subitem_publisher":"National Agriculture and Food Research Organization, Japan (NARO)"}]},"item_10_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.34503/naroj.2021.9_51","subitem_relation_type_select":"DOI"}}]},"item_10_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"本文はJ-Stageよりご利用いただけます。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This article full-text PDF is available at J-Stage."}]}]},"item_10_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.34503/naroj.2021.9_51"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.34503/naroj.2021.9_51","subitem_relation_type_select":"DOI"}}]},"item_10_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"2022.2 J-Stageへ移管しました"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"We changed the journal platform to J-Stage.　2022.2 -"}]}]},"item_10_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2434-9909","subitem_source_identifier_type":"ISSN"}]},"item_10_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"一法師, 克成"},{"creatorName":"イッポウシ, カツナリ","creatorNameLang":"ja-Kana"},{"creatorName":"IPPOUSHI, Katsunari","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_login","displaytype":"detail","filename":"10.24514-00006681_DOI_moved.pdf","filesize":[{"value":"279.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"10.24514-00006681_DOI_moved","url":"https://repository.naro.go.jp/record/6826/files/10.24514-00006681_DOI_moved.pdf"},"version_id":"ebf58bf6-9425-4b61-ac83-fefa51ca23f2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"β-conglycinin","subitem_subject_scheme":"Other"},{"subitem_subject":"soybean","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycine max (L.) Merr.","subitem_subject_scheme":"Other"},{"subitem_subject":"liquid chromatography/tandem mass spectrometry (LC/MS/MS)","subitem_subject_scheme":"Other"},{"subitem_subject":"stable isotope-labelled peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"β-conglycinin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"soybean","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycine max (L.) Merr.","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liquid chromatography/tandem mass spectrometry (LC/MS/MS)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"stable isotope-labelled peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method"},{"subitem_title":"Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method","subitem_title_language":"en"}]},"item_type_id":"10","owner":"11","path":["578"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-17"},"publish_date":"2021-12-17","publish_status":"0","recid":"6826","relation_version_is_last":true,"title":["Analytical assay for β-conglycinin in soybeans using cryogenic milling and the absolute quantification (AQUA) method"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-12-19T03:27:43.156849+00:00"}