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  1. INDEX:組織別
  2. 農研機構 食品研究部門
  3. 研究報告
  4. 食総研報
  5. 77号

いんげん豆あん粒子中のたんぱく質組成ならびにそのペプシン消化耐性について

https://doi.org/10.24514/00002903
https://doi.org/10.24514/00002903
95191b20-8ecb-4772-90d9-ff5efaa823d0
名前 / ファイル ライセンス アクション
nfri_report_No77p13-17p.pdf nfri_report_No77p13-17p.pdf (528.4 kB)
Item type 紀要論文01 / Departmental Bulletin Original Article(1)
公開日 2019-12-20
タイトル
タイトル いんげん豆あん粒子中のたんぱく質組成ならびにそのペプシン消化耐性について
タイトル
言語 en
タイトル Composition and Pepsin Digestibility of Proteins Extracted from Microground Particles in Cooked Bean Paste (Ann)
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 Ann particle
キーワード
言語 en
主題Scheme Other
主題 white common bean
キーワード
言語 en
主題Scheme Other
主題 legumin
キーワード
言語 en
主題Scheme Other
主題 pepsin-resistant protein
キーワード
言語 en
主題Scheme Other
主題 micro grinding
キーワード
言語 en
主題Scheme Other
主題 Abbreviations: SDS-PAGE
キーワード
言語 en
主題Scheme Other
主題 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
ID登録
ID登録 10.24514/00002903
ID登録タイプ JaLC
著者 門間, 美千子

× 門間, 美千子

WEKO 1317
e-Rad 20353956
researchmap michiko0815

門間, 美千子

ja-Kana モンマ, ミチコ

en MONMA, Michiko

Search repository
抄録
内容記述タイプ Abstract
内容記述 In Japan, common beans are often processed to a cooked bean paste (Ann) for confectionary and bakery use. It is known that processing the beans to Ann paste causes formation of cell particles called Ann particles, which are highly resistant to human digestion. In this study, we microground freeze-dried Ann paste by using a jet mill; subsequently, proteins extracted from the cell particles were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and pepsin assays. The micrographs of Ann particles showed that they had been thoroughly disintegrated by the microgrinding process. The SDS-PAGE profile indicated that several polypeptides, including the acidic and the basic subunits of legumin, had been extracted from the microground Ann particles. The microground Ann particles had a low concentration of phaseolin, which is one of the major proteins found in common beans and is present in higher quantities in bean flour. Among the proteins, polypeptides that were presumably derived from legumin subunits showed significant resistance to pepsin. These results suggested that the traditional method of bean paste cooking is associated with the formation of Ann particles that trap pepsinresistant polypeptides.
抄録
内容記述タイプ Abstract
内容記述 いんげん豆はあん等, 全粒で加熱加工し, 菓子やパン等に利用されることが多い. いんげん豆を全粒加熱したとき, 細胞由来の難消化性の粒子が形成されるとともにペプシン抵抗性のレグミン塩基性サブユニットが検出されなくなることが明らかとなっている. 本報告では, 凍結乾燥したあんをジェットミルで微粉砕し, 内部に含有されるたんぱく質組成を調べた. あん粒子微粉砕物からはレグミン塩基性サブユニットを含む数種類以上のポリペプチドが抽出され, その組成はいんげん豆たんぱく質とは異なり, 主要たんぱく質であるファゼオリンの含有量が低かった. あん粒子から抽出したたんぱく質のペプシン消化性を測定したところレグミンサブユニットに由来すると見られるポリペプチドが高いペプシン抵抗性を示した. 以上のことから, いんげん豆を全粒で加工するとペプシン消化耐性たんぱく質を包含する粒子が形成されることが示された.
書誌情報 食品総合研究所研究報告
en : Report of National Food Research Institute

巻 77, p. 13-17, 発行日 2013-03-01
出版者
出版者 農業・食品産業技術総合研究機構
ISSN
収録物識別子タイプ ISSN
収録物識別子 0301-9780
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.24514/00002903
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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