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Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence
https://doi.org/10.24514/00002841
https://doi.org/10.24514/0000284161e3126c-b5f8-42c6-bb32-0d89a88d3d3a
| 名前 / ファイル | ライセンス | アクション |
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nfri_report_No73p1-6p.pdf (127.3 kB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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| 公開日 | 2019-12-20 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence | |||||||||||
| タイトル | ||||||||||||
| タイトル | Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | LEA protein, late embryogenesis abundant protein | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | PAGE, polyacrylamide gel electrophoresis | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | PMSF, phenylmethanesulphonyl fluoride | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | PVDF, polyvinylidene difluoride | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00002841 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
門間, 美千子
× 門間, 美千子
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Dehydrin is a group 2 LEA (late embryogenesis abundant) protein that is widely found in plants under water stress and in mature plant seeds. As yet, the presence of dehydrin in buckwheat has not been reported. In this study, we detected dehydrin-like proteins in buckwheat by using immunoblotting with an antibody against dehydrin's highly conserved lysine-rich sequence. Both major (20 kDa) and minor (16 kDa) bands were found in common buckwheat flours, as well as in dried and in cooked buckwheat noodles. By using SDS-PAGE, it was difficult to recognize the major dehydrin-like proteins, since their molecular weight is very close to that of legumin (22-24 kDa), an abundant storage protein of buckwheat. In two-dimensional electrophoresis, two spots at pI 7.2 reacted with the antibody to the dehydrin motif. The minor dehydrin-like proteins at 16 kDa showed moderate resistance to pepsin digestion. From these results, it was suggested that buckwheat and its products contain dehydrin-like proteins, which might be associated with pepsin resistance and buckwheat allergy. | |||||||||||
| 書誌情報 |
食品総合研究所研究報告 en : Report of National Food Research Institute 巻 73, p. 1-6, 発行日 2009-03-01 |
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| 出版者 | ||||||||||||
| 出版者 | 農業・食品産業技術総合研究機構 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0301-9780 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00002841 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
