{"created":"2023-05-15T13:38:55.091130+00:00","id":2959,"links":{},"metadata":{"_buckets":{"deposit":"0306b802-0cf7-42ad-a001-2bfc7d253932"},"_deposit":{"created_by":12,"id":"2959","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2959"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002959","sets":["87:627:628:388:396"]},"author_link":["1317"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Dehydrin is a group 2 LEA (late embryogenesis abundant) protein that is widely found in plants under water stress and in mature plant seeds. As yet, the presence of dehydrin in buckwheat has not been reported. In this study, we detected dehydrin-like proteins in buckwheat by using immunoblotting with an antibody against dehydrin's highly conserved lysine-rich sequence. Both major (20 kDa) and minor (16 kDa) bands were found in common buckwheat flours, as well as in dried and in cooked buckwheat noodles. By using SDS-PAGE, it was difficult to recognize the major dehydrin-like proteins, since their molecular weight is very close to that of legumin (22-24 kDa), an abundant storage protein of buckwheat. In two-dimensional electrophoresis, two spots at pI 7.2 reacted with the antibody to the dehydrin motif. The minor dehydrin-like proteins at 16 kDa showed moderate resistance to pepsin digestion. From these results, it was suggested that buckwheat and its products contain dehydrin-like proteins, which might be associated with pepsin resistance and buckwheat allergy.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002841","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002841","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"門間, 美千子"},{"creatorName":"モンマ, ミチコ","creatorNameLang":"ja-Kana"},{"creatorName":"MONMA, Michiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-04"}],"displaytype":"detail","filename":"nfri_report_No73p1-6p.pdf","filesize":[{"value":"127.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No73p1-6p.pdf","url":"https://repository.naro.go.jp/record/2959/files/nfri_report_No73p1-6p.pdf"},"version_id":"6b7cbf2e-7b18-4e86-9ab0-4285d7a44c15"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LEA protein, late embryogenesis abundant protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PAGE, polyacrylamide gel electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PMSF, phenylmethanesulphonyl fluoride","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PVDF, polyvinylidene difluoride","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence"},{"subitem_title":"Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["396"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2959","relation_version_is_last":true,"title":["Detection of dehydrin-like proteins in buckwheat by immunoblotting with an antibody against the lysine-rich sequence"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:52:02.354360+00:00"}