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Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success
https://repository.naro.go.jp/records/184
https://repository.naro.go.jp/records/1849ea68c5e-58fb-465d-aec0-73e21fc32907
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC10201707100005_CCBYNCND_62_2016-103.pdf (905.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2018-02-05 | |||||||
| タイトル | ||||||||
| タイトル | Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success | |||||||
| タイトル | ||||||||
| タイトル | Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Fertilization | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Intracytoplasmic sperm injection | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Oocyte activation | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Pig | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Fertilization | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Intracytoplasmic sperm injection | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Oocyte activation | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Pig | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 著者 |
NAKAI, Michiko
× NAKAI, Michiko× SUZUKI, Shun-ichi× ITO, Junya× FUCHIMOTO, Daiichiro× SENBON, Shoichiro× NOGUCHI, Junko× Onishi, Akira× KASHIWAZAKI, Naomi× KIKUCHI, Kazuhiro
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injection (ICSI) using Percoll-selected sperm can be improved. Percoll-selected sperm (Percoll group) or sperm without Percoll selection (Control group) were used. A proportion of the oocytes injected with control sperm were subjected to electrical stimulation at 1 h after ICSI (Cont + ES group). It was found that the Percoll group showed a large amount of PLCζ in comparison with the Control group. Furthermore, application of Percoll-selected sperm for ICSI increased the efficiency of fertilization and embryo development. Thus, these results indicate the Percoll-selected sperm have sufficient PLCζ and high oocyte-activating ability after ICSI in pigs. | |||||||
| 書誌情報 |
Journal of Reproduction and Development en : Journal of Reproduction and Development 巻 62, 号 6, p. 639-643, 発行日 2016 |
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| 出版者 | ||||||||
| 出版者 | Society for Reproduction and Development | |||||||
| 出版者 | ||||||||
| 出版者 | 日本繁殖生物学会 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1348-4400 | |||||||
| PubMed番号 | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 27725346 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1262/jrd.2016-103 | |||||||
| 権利 | ||||||||
| 権利情報 | (c) 2016 Society for Reproduction and Development | |||||||
| 権利 | ||||||||
| 権利情報 | (c) 2016 日本繁殖生物学会 | |||||||
| 情報源 | ||||||||
| 関連名称 | SC10201707100005 | |||||||
| 関連サイト | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | http://dx.doi.org/10.1262/jrd.2016-103 | |||||||
| 関連名称 | Journal of Reproduction and Development | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
