{"created":"2023-05-15T13:36:41.599955+00:00","id":184,"links":{},"metadata":{"_buckets":{"deposit":"09ad837e-4876-4f9f-b708-a14b1b1ac721"},"_deposit":{"created_by":11,"id":"184","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"184"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000184","sets":["2:581:62","87:654:46"]},"author_link":["1754","1481","4865","701","952","2560","3694","1831","1095"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"643","bibliographicPageStart":"639","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"Journal of Reproduction and Development"},{"bibliographic_title":"Journal of Reproduction and Development","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injection (ICSI) using Percoll-selected sperm can be improved. Percoll-selected sperm (Percoll group) or sperm without Percoll selection (Control group) were used. A proportion of the oocytes injected with control sperm were subjected to electrical stimulation at 1 h after ICSI (Cont + ES group). It was found that the Percoll group showed a large amount of PLCζ in comparison with the Control group. Furthermore, application of Percoll-selected sperm for ICSI increased the efficiency of fertilization and embryo development. Thus, these results indicate the Percoll-selected sperm have sufficient PLCζ and high oocyte-activating ability after ICSI in pigs.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Reproduction and Development"},{"subitem_publisher":"日本繁殖生物学会 "}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"27725346","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1262/jrd.2016-103","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC10201707100005"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Reproduction and Development"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1262/jrd.2016-103","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2016 Society for Reproduction and Development"},{"subitem_rights":"(c) 2016 日本繁殖生物学会 "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-4400","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"NAKAI, Michiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"SUZUKI, Shun-ichi ","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"ITO, Junya ","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"FUCHIMOTO, Daiichiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"SENBON, Shoichiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"NOGUCHI, Junko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Onishi, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KASHIWAZAKI, Naomi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KIKUCHI, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-05"}],"displaytype":"detail","filename":"SC10201707100005_CCBYNCND_62_2016-103.pdf","filesize":[{"value":"905.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC10201707100005_CCBYNCND_62_2016-103.pdf","url":"https://repository.naro.go.jp/record/184/files/SC10201707100005_CCBYNCND_62_2016-103.pdf"},"version_id":"08acac84-cedb-45f5-bad4-940f34605c28"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fertilization","subitem_subject_scheme":"Other"},{"subitem_subject":"Intracytoplasmic sperm injection","subitem_subject_scheme":"Other"},{"subitem_subject":"Oocyte activation","subitem_subject_scheme":"Other"},{"subitem_subject":"Pig","subitem_subject_scheme":"Other"},{"subitem_subject":"Fertilization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Intracytoplasmic sperm injection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oocyte activation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pig","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success"},{"subitem_title":"Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["46","62"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-05"},"publish_date":"2018-02-05","publish_status":"0","recid":"184","relation_version_is_last":true,"title":["Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T17:05:01.020452+00:00"}