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Random Amplification DNA Fingerprintingによる牛乳房炎主要原因菌種の同定
https://doi.org/10.24514/00001662
https://doi.org/10.24514/00001662c8a427cb-e7f8-4718-ae69-c0ce30890744
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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公開日 | 2019-03-22 | |||||||||||
タイトル | ||||||||||||
タイトル | Random Amplification DNA Fingerprintingによる牛乳房炎主要原因菌種の同定 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Application of random amplification DNA fingerprinting for identification of bacteria isolated from bovine mastitis | |||||||||||
言語 | ||||||||||||
言語 | jpn | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | departmental bulletin paper | |||||||||||
ID登録 | ||||||||||||
ID登録 | 10.24514/00001662 | |||||||||||
ID登録タイプ | JaLC | |||||||||||
著者 |
勝田, 賢
× 勝田, 賢× 江口, 正志× 坪井, 孝益× 横山, 隆
WEKO
2168
× 西森, 敬× 内田, 郁夫× 田中, 聖× 西森, 知子× 今井, 邦俊 |
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | A polymerase chain reaction-based DNA fingerprinting system for species identification of bacteria isolated from mastitic milk was developed using random amplified polymorphic DNA. A total of 662 isolates belonging to coliforms, Staphylococcus, Streptococcus, and Enterococcus species were used to develop the system. It is apparent from the present study that primer OEP-04 generated DNA fragments that can be used for differentiation and identification of members of the genera Staphylococcus, Streptococcus and Enterococcus species. The RAPD analysis of coliforms species using primer AP-45 has shown promising results for differentiation and identification of those bacteria. This technique is cost effective, simple to perform and may be adopted as a fast and easy identification technique by other laboratories involved in diagnosis of mastitis pathogens when a dedicated analysis software is developed in the future. | |||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Random Amplification DNA Fingerprinting(RAPD)法により乳房炎主要原因菌であるブドウ球菌, レンサ状球菌, 大腸菌群の同定方法を検討した. 乳房炎乳汁から分離されたレンサ状球菌(8菌種;296株), ブドウ球菌(9菌種;215株), 大腸菌群(8菌種;151株)を市販簡易同定キットで同定し, 選定したプライマーを用いてRAPD法を実施した. その結果, ブドウ球菌およびレンサ状球菌の菌種識別に有望なプライマーOEP-04(gTgACATgCC), 大腸菌群の菌種識別に有望なプライマーAP-45(gTCAACgAAg)を得ることができた. 供試レンサ状球菌は全て識別されたが, 供試ブドウ球菌中Staphylococcus hyicus とStaphylococcus simulans は識別できなかった. しかし, これら2菌種は他の供試ブドウ球菌と識別された. 供試大腸菌群のうちSerrataia marcescens は菌種に共通な遺伝子増幅産物は認められず, この菌種の識別に選定したプライマーによるRAPD法の応用は不可能と考えられたが, 他の7菌種はRAPD法により識別可能であった. 以上のことから, 一部の例外を除き, RAPD法により乳房炎主要菌種の同定は可能と考えられる. | |||||||||||
書誌情報 |
動物衛生研究所研究報告 en : Bulletin of the National Institute of Animal Health 巻 108, p. 1-7, 発行日 2002-03-31 |
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出版者 | ||||||||||||
出版者 | 独立行政法人 農業技術研究機構 動物衛生研究所 | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 1347-2542 | |||||||||||
DOI | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | 10.24514/00001662 | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |