{"_buckets": {"deposit": "9c1bcdd3-058f-4160-8287-557fa9aeaeca"}, "_deposit": {"created_by": 11, "id": "1706", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "1706"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00001706", "sets": ["327"]}, "author_link": ["5846", "2168", "5840", "5851", "5858", "1020", "5860", "899", "2976"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-03-31", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "7", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "108", "bibliographic_titles": [{"bibliographic_title": "動物衛生研究所研究報告"}, {"bibliographic_title": "Bulletin of the National Institute of Animal Health", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A polymerase chain reaction-based DNA fingerprinting system for species identification of bacteria isolated from mastitic milk was developed using random amplified polymorphic DNA. A total of 662 isolates belonging to coliforms, Staphylococcus, Streptococcus, and Enterococcus species were used to develop the system. It is apparent from the present study that primer OEP-04 generated DNA fragments that can be used for differentiation and identification of members of the genera Staphylococcus, Streptococcus and Enterococcus species. The RAPD analysis of coliforms species using primer AP-45 has shown promising results for differentiation and identification of those bacteria. This technique is cost effective, simple to perform and may be adopted as a fast and easy identification technique by other laboratories involved in diagnosis of mastitis pathogens when a dedicated analysis software is developed in the future.", "subitem_description_type": "Abstract"}, {"subitem_description": "Random Amplification DNA Fingerprinting（RAPD）法により乳房炎主要原因菌であるブドウ球菌, レンサ状球菌, 大腸菌群の同定方法を検討した. 乳房炎乳汁から分離されたレンサ状球菌（8菌種；296株）, ブドウ球菌（9菌種；215株）, 大腸菌群（8菌種；151株）を市販簡易同定キットで同定し, 選定したプライマーを用いてRAPD法を実施した. その結果, ブドウ球菌およびレンサ状球菌の菌種識別に有望なプライマーOEP-04（gTgACATgCC）, 大腸菌群の菌種識別に有望なプライマーAP-45（gTCAACgAAg）を得ることができた. 供試レンサ状球菌は全て識別されたが, 供試ブドウ球菌中Staphylococcus hyicus とStaphylococcus simulans は識別できなかった. しかし, これら2菌種は他の供試ブドウ球菌と識別された. 供試大腸菌群のうちSerrataia marcescens は菌種に共通な遺伝子増幅産物は認められず, この菌種の識別に選定したプライマーによるRAPD法の応用は不可能と考えられたが, 他の7菌種はRAPD法により識別可能であった. 以上のことから, 一部の例外を除き, RAPD法により乳房炎主要菌種の同定は可能と考えられる. ", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24514/00001662", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "独立行政法人 農業技術研究機構 動物衛生研究所"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.24514/00001662 ", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1347-2542", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": 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"en"}]}, "item_type_id": "10002", "owner": "11", "path": ["327"], "permalink_uri": "https://doi.org/10.24514/00001662", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-03-22"}, "publish_date": "2019-03-22", "publish_status": "0", "recid": "1706", "relation": {}, "relation_version_is_last": true, "title": ["Random Amplification DNA Fingerprintingによる牛乳房炎主要原因菌種の同定"], "weko_shared_id": -1}