{"created":"2023-05-15T13:37:58.510399+00:00","id":1706,"links":{},"metadata":{"_buckets":{"deposit":"9c1bcdd3-058f-4160-8287-557fa9aeaeca"},"_deposit":{"created_by":11,"id":"1706","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"1706"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001706","sets":["87:639:640:134:327"]},"author_link":["5846","2168","5840","5851","5858","1020","5860","899","2976"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"108","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A polymerase chain reaction-based DNA fingerprinting system for species identification of bacteria isolated from mastitic milk was developed using random amplified polymorphic DNA. A total of 662 isolates belonging to coliforms, Staphylococcus, Streptococcus, and Enterococcus species were used to develop the system. It is apparent from the present study that primer OEP-04 generated DNA fragments that can be used for differentiation and identification of members of the genera Staphylococcus, Streptococcus and Enterococcus species. The RAPD analysis of coliforms species using primer AP-45 has shown promising results for differentiation and identification of those bacteria. This technique is cost effective, simple to perform and may be adopted as a fast and easy identification technique by other laboratories involved in diagnosis of mastitis pathogens when a dedicated analysis software is developed in the future.","subitem_description_type":"Abstract"},{"subitem_description":"Random Amplification DNA Fingerprinting（RAPD）法により乳房炎主要原因菌であるブドウ球菌, レンサ状球菌, 大腸菌群の同定方法を検討した. 乳房炎乳汁から分離されたレンサ状球菌（8菌種；296株）, ブドウ球菌（9菌種；215株）, 大腸菌群（8菌種；151株）を市販簡易同定キットで同定し, 選定したプライマーを用いてRAPD法を実施した. その結果, ブドウ球菌およびレンサ状球菌の菌種識別に有望なプライマーOEP-04（gTgACATgCC）, 大腸菌群の菌種識別に有望なプライマーAP-45（gTCAACgAAg）を得ることができた. 供試レンサ状球菌は全て識別されたが, 供試ブドウ球菌中Staphylococcus hyicus とStaphylococcus simulans は識別できなかった. しかし, これら2菌種は他の供試ブドウ球菌と識別された. 供試大腸菌群のうちSerrataia marcescens は菌種に共通な遺伝子増幅産物は認められず, この菌種の識別に選定したプライマーによるRAPD法の応用は不可能と考えられたが, 他の7菌種はRAPD法により識別可能であった. 以上のことから, 一部の例外を除き, RAPD法により乳房炎主要菌種の同定は可能と考えられる. ","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001662","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業技術研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001662 ","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"勝田, 賢"},{"creatorName":"カツダ, ケン","creatorNameLang":"ja-Kana"},{"creatorName":"KATSUDA, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"江口, 正志"},{"creatorName":"エグチ, マサシ","creatorNameLang":"ja-Kana"},{"creatorName":"EGUCHI, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"坪井, 孝益"},{"creatorName":"ツボイ, タカミツ","creatorNameLang":"ja-Kana"},{"creatorName":"TSUBOI, Takamitsu","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"横山, 隆"},{"creatorName":"ヨコヤマ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"YOKOYAMA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"西森, 敬"},{"creatorName":"ニシモリ, ケイ","creatorNameLang":"ja-Kana"},{"creatorName":"NISHIMORI, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"内田, 郁夫"},{"creatorName":"ウチダ, イクオ","creatorNameLang":"ja-Kana"},{"creatorName":"UCHIDA, Ikuo","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"田中, 聖"},{"creatorName":"タナカ, キヨシ","creatorNameLang":"ja-Kana"},{"creatorName":"TANAKA, Kiyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西森, 知子"},{"creatorName":"ニシモリ, トモコ","creatorNameLang":"ja-Kana"},{"creatorName":"NISHIMORI, Tomoko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"今井, 邦俊"},{"creatorName":"イマイ, クニトシ","creatorNameLang":"ja-Kana"},{"creatorName":"IMAI, Kunitoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-18"}],"displaytype":"detail","filename":"niah_report_No108p1-7p.pdf","filesize":[{"value":"45.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No108p1-7p","url":"https://repository.naro.go.jp/record/1706/files/niah_report_No108p1-7p.pdf"},"version_id":"6da51e4c-490d-417e-938c-90d51926778d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Random Amplification DNA Fingerprintingによる牛乳房炎主要原因菌種の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Random Amplification DNA Fingerprintingによる牛乳房炎主要原因菌種の同定"},{"subitem_title":"Application of random amplification DNA fingerprinting for identification of bacteria isolated from bovine mastitis","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["327"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1706","relation_version_is_last":true,"title":["Random Amplification DNA Fingerprintingによる牛乳房炎主要原因菌種の同定"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T15:28:50.694846+00:00"}