| アイテムタイプ |
紀要論文01 / Departmental Bulletin Original Article(1) |
| 公開日 |
2019-03-22 |
| タイトル |
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タイトル |
サテライト RNA 遺伝子の導入によるキュウリモザイクウイルス抵抗性トマトに関する研究 : 第3報 サテライト RNA の転写および伝搬による変異性の解明 |
| タイトル |
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タイトル |
Virus Resistance in Transgenic Tomato Expressing Satellite RNA of Cucumber mosaic virus : 3 : Mutation Analysis of Satellite RNA through Translation and Transmission |
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言語 |
en |
| 言語 |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| ID登録 |
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ID登録 |
10.24514/00001346 |
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ID登録タイプ |
JaLC |
| 著者 |
眞岡, 哲夫
早野-斎藤, 由里子
| ja |
早野-斎藤, 由里子
早野, 由里子
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| ja-Kana |
ハヤノ-サイトウ, ユリコ
ハヤノ, ユリコ
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| en |
HAYANO-SAITO, Yuriko
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Search repository
河辺, 邦正
福本, 文良
柏崎, 哲
岩崎, 眞人
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
A transgenic tomato line, "No.4-7", expressing a satellite RNA 55-1 of Cucumber mosaic virus (CMV), which shows resistance to CMV, has been developed. We previously reported the genetics and virus resistance of "No.4-7" (KAWABE et al., 2002) and also results of evaluation of the biosafety of transgenic tomato "No.4-7" in terms of influence on agricultural traits and the environment (IWASAKI et al., 2005). This is the third and last report on the transgenic tomato line "No.4-7". We passaged a satellite RNA 55-1 of CMV with its helper virus to determine whether a satellite RNA that attenuated CMV-induced disease on tobacco or tomato plants could mutate to a pathogenic form. No remarkable symptom was observed on the test plants after 10 passages. The sequence diversity of satellite RNA 55-1 of "No.4-7" was checked in various cases : genomic DNA, its transcribed RNA, satellite RNA repackaged in a helper virus, satellite RNA with a helper virus transmitted into other plants by an aphid, and satellite RNA in a plant for a long period. The rate of mutation in the sequence of satellite RNA 55-1 was from 0 to 1.9%, and no change of pathogenicity from attenuate to severe was observed. These results prove the biosafety of the transgenic tomato line "No.4-7". Guidelines for assessment of the biosafety of a satellite RNA are also proposed. |
| 書誌情報 |
北海道農業研究センター研究報告
en : Research BUlletin of the NARO Hokkaido Agricultural Research Center
巻 188,
p. 1-28,
発行日 2008-03-01
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| 出版者 |
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出版者 |
独立行政法人 農業・食品産業技術総合研究機構 北海道農業研究センター |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1347-8117 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.24514/00001346 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
harc_report_No188_02.pdf (1.9 MB)
