@article{oai:repository.naro.go.jp:00001381,
 author = {眞岡, 哲夫 and MAOKA, Tetsuo and 早野-斎藤, 由里子 and HAYANO-SAITO, Yuriko and 早野, 由里子 and 河辺, 邦正 and KAWABE, Kunimasa and 福本, 文良 and FUKUMOTO, Fumiyoshi and 柏崎, 哲 and KASHIWAZAKI, Satoshi and 岩崎, 眞人 and IWASAKI, Mabito},
 journal = {北海道農業研究センター研究報告, Research BUlletin of the NARO Hokkaido Agricultural Research Center},
 month = {Mar},
 note = {A transgenic tomato line, "No.4-7", expressing a satellite RNA 55-1 of Cucumber mosaic virus (CMV), which shows resistance to CMV, has been developed. We previously reported the genetics and virus resistance of "No.4-7" (KAWABE et al., 2002) and also results of evaluation of the biosafety of transgenic tomato "No.4-7" in terms of influence on agricultural traits and the environment (IWASAKI et al., 2005). This is the third and last report on the transgenic tomato line "No.4-7". We passaged a satellite RNA 55-1 of CMV with its helper virus to determine whether a satellite RNA that attenuated CMV-induced disease on tobacco or tomato plants could mutate to a pathogenic form. No remarkable symptom was observed on the test plants after 10 passages. The sequence diversity of satellite RNA 55-1 of "No.4-7" was checked in various cases : genomic DNA, its transcribed RNA, satellite RNA repackaged in a helper virus, satellite RNA with a helper virus transmitted into other plants by an aphid, and satellite RNA in a plant for a long period. The rate of mutation in the sequence of satellite RNA 55-1 was from 0 to 1.9%, and no change of pathogenicity from attenuate to severe was observed. These results prove the biosafety of the transgenic tomato line "No.4-7". Guidelines for assessment of the biosafety of a satellite RNA are also proposed.},
 pages = {1--28},
 title = {サテライト RNA 遺伝子の導入によるキュウリモザイクウイルス抵抗性トマトに関する研究 : 第3報 サテライト RNA の転写および伝搬による変異性の解明},
 volume = {188},
 year = {2008},
 yomi = {マオカ, テツオ and ハヤノ-サイトウ, ユリコ and ハヤノ, ユリコ and カワベ, クニマサ and フクモト, フミヨシ and カシワザキ, サトシ and イワサキ, マビト}
}