| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-03-29 |
| タイトル |
|
|
タイトル |
Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm |
| タイトル |
|
|
タイトル |
Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
trehalose |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
DNA fragmentation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
freeze-drying |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
ICSI |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
pig |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
trehalose |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA fragmentation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
freeze-drying |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ICSI |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
pig |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
ニュエン, テイ・メン
菊地, 和弘
古澤, 軌
ダン・グェン, タィン・クァン
中井, 美智子
福田, 篤徳
野口, 純子
金子, 浩之
Nguyen, Viet Linh
Bui, Xuan Nguyen
田島, 淳史
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Boar sperm freeze‐dried with trehalose showed a protective effect against sperm DNA fragmentation. However, normal fertilization and embryonic development were not improved. Damaged sperm may activate maternal DNA repair genes when injected into oocytes. Therefore, we investigated the expression profile of some DNA repair genes in porcine oocytes after intra‐cytoplasmic sperm injection. First, the expression levels of MGMT, UDG, XPC, MSH2, XRCC6 and RAD51 genes that are concerned with different types of DNA repair were examined in in vitro mature (IVM) oocytes injected with ejaculated sperm, or freeze‐dried sperm with or without trehalose. Quantitative reverse transcription polymerase chain reaction revealed that expression of six DNA repair genes in the oocytes at 4 h after injection did not differ among the four groups. Next, we investigated the gene expression levels of these genes at different stages of maturation. The relative expression levels of UDG and XPC were significantly up‐regulated in mature oocytes compared with earlier stages. Furthermore, there was an increased tendency in relative expression of MSH2 and RAD51. These results suggested two possible mechanisms that messenger RNA of DNA repair genes are either accumulated during IVM to be ready for fertilization or increased expression levels of DNA repair genes in oocytes caused by suboptimal IVM conditions. |
| 書誌情報 |
Animal Science Journal
en : Animal Science Journal
巻 87,
号 11,
p. 1325-1333,
発行日 2016-10-23
|
| 出版者 |
|
|
出版者 |
John Wiley & Sons, Inc. |
| 出版者 |
|
|
出版者 |
Japanese Society of Animal Science |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1740-0929 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
26988944 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1111/asj.12554 |
| 権利 |
|
|
権利情報 |
This is the peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/abs/10.1111/asj.12554], which has been published in final form at http://dx.doi.org/10.1111/asj.12554]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| 権利 |
|
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご利用ください。 |
| 権利 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 情報源 |
|
|
|
関連名称 |
SC10201707060027 |
| 関連サイト |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1111/asj.12554 |
|
|
関連名称 |
Animal Science Journal |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
SC10201707060027_PostPrint (448.6 kB)
.png)
