{"created":"2023-05-15T13:36:45.694801+00:00","id":270,"links":{},"metadata":{"_buckets":{"deposit":"b1a97529-e7af-45aa-b272-4631879a25ce"},"_deposit":{"created_by":11,"id":"270","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"270"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000270","sets":["2:581:69","87:647:47","87:654:46"]},"author_link":["1481","4863","680","2911","6071","2910","998","952","480","2030","1095"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"1333","bibliographicPageStart":"1325","bibliographicVolumeNumber":"87","bibliographic_titles":[{"bibliographic_title":"Animal Science Journal"},{"bibliographic_title":"Animal Science Journal","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Boar sperm freeze‐dried with trehalose showed a protective effect against sperm DNA fragmentation. However, normal fertilization and embryonic development were not improved. Damaged sperm may activate maternal DNA repair genes when injected into oocytes. Therefore, we investigated the expression profile of some DNA repair genes in porcine oocytes after intra‐cytoplasmic sperm injection. First, the expression levels of MGMT, UDG, XPC, MSH2, XRCC6 and RAD51 genes that are concerned with different types of DNA repair were examined in in vitro mature (IVM) oocytes injected with ejaculated sperm, or freeze‐dried sperm with or without trehalose. Quantitative reverse transcription polymerase chain reaction revealed that expression of six DNA repair genes in the oocytes at 4 h after injection did not differ among the four groups. Next, we investigated the gene expression levels of these genes at different stages of maturation. The relative expression levels of UDG and XPC were significantly up‐regulated in mature oocytes compared with earlier stages. Furthermore, there was an increased tendency in relative expression of MSH2 and RAD51. These results suggested two possible mechanisms that messenger RNA of DNA repair genes are either accumulated during IVM to be ready for fertilization or increased expression levels of DNA repair genes in oocytes caused by suboptimal IVM conditions. ","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons, Inc."},{"subitem_publisher":"Japanese Society of Animal Science"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"26988944","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/asj.12554","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC10201707060027"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Animal Science Journal"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1111/asj.12554","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is the peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/abs/10.1111/asj.12554], which has been published in final form at http://dx.doi.org/10.1111/asj.12554]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."},{"subitem_rights":"この論文は出版社版ではありません。引用の際には出版社版をご利用ください。"},{"subitem_rights":"This is not the published version. Please cite only the published version. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1740-0929","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ニュエン, テイ・メン"},{"creatorName":"ニュエン, テイ・メン","creatorNameLang":"ja-Kana"},{"creatorName":"NGUYEN, Thi Men","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4863","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菊地, 和弘"},{"creatorName":"キクチ, カズヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"KIKUCHI, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1095","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20360456","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20360456"},{"nameIdentifier":"read0004406","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0004406"},{"nameIdentifier":"0000-0002-7198-9237","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0002-7198-9237"}]},{"creatorNames":[{"creatorName":"古澤, 軌"},{"creatorName":"フルサワ, タダシ","creatorNameLang":"ja-Kana"},{"creatorName":"FURUSAWA, Tadashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2030","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00343997","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00343997"},{"nameIdentifier":"read0080254","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0080254"}]},{"creatorNames":[{"creatorName":"ダン・グェン, タィン・クァン"},{"creatorName":"ダン・グェン, タィン・クァン","creatorNameLang":"ja-Kana"},{"creatorName":"DANG-NGUYEN, Than Quang","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"480","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40770128","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40770128"},{"nameIdentifier":"adipy","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/adipy"},{"nameIdentifier":"0000-0001-8176-4827","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0001-8176-4827"}]},{"creatorNames":[{"creatorName":"中井, 美智子"},{"creatorName":"ナカイ, ミチコ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAI, Michiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1481","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30442825","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30442825"},{"nameIdentifier":"pig-fertilization","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/pig-fertilization"}]},{"creatorNames":[{"creatorName":"福田, 篤徳"},{"creatorName":"フクダ, アツノリ","creatorNameLang":"ja-Kana"},{"creatorName":"FUKUDA, Atsunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"680","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40414940","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40414940"},{"nameIdentifier":"read0004393","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0004393"}]},{"creatorNames":[{"creatorName":"野口, 純子"},{"creatorName":"ノグチ, ジュンコ","creatorNameLang":"ja-Kana"},{"creatorName":"NOGUCHI, Junko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"952","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80189381","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80189381"},{"nameIdentifier":"read0004422","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0004422"}]},{"creatorNames":[{"creatorName":"金子, 浩之"},{"creatorName":"カネコ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"KANEKO, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"998","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60343993","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60343993"},{"nameIdentifier":"read0084939","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0084939"},{"nameIdentifier":"0000-0003-1829-3895","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0003-1829-3895"}]},{"creatorNames":[{"creatorName":"Nguyen, Viet Linh "},{"creatorName":"Nguyen, Viet Linh ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bui, Xuan Nguyen"},{"creatorName":"Bui, Xuan Nguyen","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田島, 淳史"},{"creatorName":"タジマ, アツシ","creatorNameLang":"ja-Kana"},{"creatorName":"TAJIMA, Atsushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6071","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"read0062190","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0062190"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-04"}],"displaytype":"detail","filename":"SC10201707060027_PostPrint_Men_Text-cleaned_15-0821.pdf","filesize":[{"value":"448.6 kB"}],"format":"application/pdf","licensetype":"license_9","mimetype":"application/pdf","url":{"label":"SC10201707060027_PostPrint","url":"https://repository.naro.go.jp/record/270/files/SC10201707060027_PostPrint_Men_Text-cleaned_15-0821.pdf"},"version_id":"76413ce7-de61-4c2e-922a-b3efc551a7cc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"trehalose","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA fragmentation","subitem_subject_scheme":"Other"},{"subitem_subject":"freeze-drying","subitem_subject_scheme":"Other"},{"subitem_subject":"ICSI","subitem_subject_scheme":"Other"},{"subitem_subject":"pig","subitem_subject_scheme":"Other"},{"subitem_subject":"trehalose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA fragmentation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"freeze-drying","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ICSI","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pig","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm"},{"subitem_title":"Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["46","47","69"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-29"},"publish_date":"2018-03-29","publish_status":"0","recid":"270","relation_version_is_last":true,"title":["Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T23:01:39.709038+00:00"}