@article{oai:repository.naro.go.jp:00000270,
 author = {ニュエン, テイ・メン and NGUYEN, Thi Men and 菊地, 和弘 and KIKUCHI, Kazuhiro and 古澤, 軌 and FURUSAWA, Tadashi and ダン・グェン, タィン・クァン and DANG-NGUYEN, Than Quang and 中井, 美智子 and NAKAI, Michiko and 福田, 篤徳 and FUKUDA, Atsunori and 野口, 純子 and NOGUCHI, Junko and 金子, 浩之 and KANEKO, Hiroyuki and Nguyen, Viet Linh and Nguyen, Viet Linh and Bui, Xuan Nguyen and Bui, Xuan Nguyen and 田島, 淳史 and TAJIMA, Atsushi},
 issue = {11},
 journal = {Animal Science Journal, Animal Science Journal},
 month = {Oct},
 note = {Boar sperm freeze‐dried with trehalose showed a protective effect against sperm DNA fragmentation. However, normal fertilization and embryonic development were not improved. Damaged sperm may activate maternal DNA repair genes when injected into oocytes. Therefore, we investigated the expression profile of some DNA repair genes in porcine oocytes after intra‐cytoplasmic sperm injection. First, the expression levels of MGMT, UDG, XPC, MSH2, XRCC6 and RAD51 genes that are concerned with different types of DNA repair were examined in in vitro mature (IVM) oocytes injected with ejaculated sperm, or freeze‐dried sperm with or without trehalose. Quantitative reverse transcription polymerase chain reaction revealed that expression of six DNA repair genes in the oocytes at 4 h after injection did not differ among the four groups. Next, we investigated the gene expression levels of these genes at different stages of maturation. The relative expression levels of UDG and XPC were significantly up‐regulated in mature oocytes compared with earlier stages. Furthermore, there was an increased tendency in relative expression of MSH2 and RAD51. These results suggested two possible mechanisms that messenger RNA of DNA repair genes are either accumulated during IVM to be ready for fertilization or increased expression levels of DNA repair genes in oocytes caused by suboptimal IVM conditions.},
 pages = {1325--1333},
 title = {Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm},
 volume = {87},
 year = {2016},
 yomi = {ニュエン, テイ・メン and キクチ, カズヒロ and フルサワ, タダシ and ダン・グェン, タィン・クァン and ナカイ, ミチコ and フクダ, アツノリ and ノグチ, ジュンコ and カネコ, ヒロユキ and タジマ, アツシ}
}