{"_buckets": {"deposit": "a0321a89-0bc2-4430-a6cf-ba61bf496e4f"}, "_deposit": {"created_by": 12, "id": "4856", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "4856"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00004856", "sets": ["517"]}, "author_link": ["7232", "7235", "2116", "1620"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-02", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "167", "bibliographicPageStart": "153", "bibliographicVolumeNumber": "28", "bibliographic_titles": [{"bibliographic_title": "植物遺伝資源探索導入調査報告書"}, {"bibliographic_title": "Annual report on exploration and introduction of plant genetic resources", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Guineagrass (Panicum maximum Jacq.) is a one of most important tropical forage grass species. This grass exhibits apomixis (asexual) propagation through seeds, this poses difficulties for developing improved cultivars by breeding. Therefore, numerous accessions have been collected and characterized, and many cultivars have been released directly using apomictic accessions. Although guineagrass has been collected and genetically analyzed, the key factor in guineagrass improvement is finding sexual lines. Phylogenetic analysis of guineagrass reveals that the Kenya is a center of diversity for guineagrass, therefore, it is thought sexual lines may exist there. A molecular marker selection method has been developed to identify sexual plantlets without the need for cytological analysis or progeny tests. Although numerous apomictic accessions have been collected, only a few sexual lines have been identified, and these do not have superior agronomical characteristics. This narrow genetic base has prevented recurrent selection of sexual lines themselves and also breeding and crossing with superior apomictic lines. Thus, agronomical superior sexual lines are required. This visit to Kenya was conducted to initiate a new search for sexual varieties of guineagrass in its native habitat. Based on observations made there appears to be the possibility of finding abundant sexual lines. During this preliminary expedition, a total of 14 native P. maximum sites were found and recorded. At each site, several morphological varieties were recorded. The Kenyan Genebank has already collected 1300 accessions of guineagrass and we hope to identify new sexual lines among these accessions. We recognized also that KARI-Katumani has 56 accessions from dividing tillers of native guineagrass, these materials should also be checked for new sexual lines.\n本報告は，世界の熱帯圏で広く牧草として利用されているギニアグラス (Panicum maximum Jacq.) の数少ない野生の有性生殖個体を発見するための予備調査の結果を示している．ギニアグラスはアポミクシスであり，母株と同じ遺伝子型の無性生殖種子で繁殖するため，交雑による育種が困難で，かつ野生の有性生殖個体は現在までのところ全世界で数系統しか発見されていない．そのため，アポミクシス自生株の収集が盛んに行われてきており，これらには豊富な自然変異が含まれている．これらの豊富な変異は自然交雑による変異と考えられ，有性生殖個体の存在を裏付けるものである．しかしながら，これまで収集されてきた有性生殖個体が少ないため，はっきりしない点が多い．豊富な変異を含むアポミクシス自生株の遺伝変異解析から，ケニアに最も豊富な変異が含まれることがこれまでの研究から明らかになってきた．また，近年DNA を用いた直接的でかつ少量の葉からすぐに判定が可能な手法が開発されてきた．そのため，ケニアの自生状況を確認するとともに，DNAの簡易判定手法の応用の可能性を検討するため，探索を行った．その結果，同一収集地点または近接収集地点で，形態的に明らかに遺伝変異を含んでいると考えられる個体を複数の地点で確認することができた．このことは，有性生殖株の存在を強く示唆するものであり，DNA簡易判別手法の応用により即座な判定を行うことができれば，希少な野生有生殖株を収集蓄積することが可能であり，これらは，今後のギニアグラスの牧草あるいはエネルギー作物としての育種の重要な基盤となることが期待される．", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24514/00004719", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "独立行政法人農業生物資源研究所"}, {"subitem_publisher": "National Institute of Agrobiological Sciences"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.24514/00004719", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2434-7485", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "蝦名, 真澄"}, {"creatorName": "エビナ, マスミ", "creatorNameLang": "ja-Kana"}, {"creatorName": "EBINA, Masumi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2116", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80355122", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80355122"}, {"nameIdentifier": "ebinam035", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/ebinam035"}]}, {"creatorNames": [{"creatorName": "MWANGI, David Miano"}, {"creatorName": "MWANGI, David Miano", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7232", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000241579230", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000241579230"}]}, {"creatorNames": [{"creatorName": "KEYA, George"}, {"creatorName": "KEYA, George", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7235", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000241579231", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000241579231"}]}, {"creatorNames": [{"creatorName": "奥泉, 久人"}, {"creatorName": "オクイズミ, ヒサト", "creatorNameLang": "ja-Kana"}, {"creatorName": "OKUIZUMI, Hisato", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1620", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20370645", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20370645"}, {"nameIdentifier": "xyz-", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/xyz-"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-11-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AREIPGR28_p153-167.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "AREIPGR28_p153-167.pdf", "url": "https://repository.naro.go.jp/record/4856/files/AREIPGR28_p153-167.pdf"}, "version_id": "04a9c539-9d21-46e9-85a7-cd37d8b5436d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Guineagrass", "subitem_subject_scheme": "Other"}, {"subitem_subject": "forage", "subitem_subject_scheme": "Other"}, {"subitem_subject": "apomixes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sexual line", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Guineagrass", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "forage", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "apomixes", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sexual line", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Collaborative Exploration for Panicum maximum Genetic Resources in Kenya, March, 2012", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Collaborative Exploration for Panicum maximum Genetic Resources in Kenya, March, 2012"}, {"subitem_title": "Collaborative Exploration for Panicum maximum Genetic Resources in Kenya, March, 2012", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "12", "path": ["517"], "permalink_uri": "https://doi.org/10.24514/00004719", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-12-04"}, "publish_date": "2020-12-04", "publish_status": "0", "recid": "4856", "relation": {}, "relation_version_is_last": true, "title": ["Collaborative Exploration for Panicum maximum Genetic Resources in Kenya, March, 2012"], "weko_shared_id": 12}