{"_buckets": {"deposit": "a0f7a123-0dc9-4ec3-8d08-3dd98b5a05af"}, "_deposit": {"created_by": 12, "id": "2797", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "2797"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00002797", "sets": ["392"]}, "author_link": ["2571", "6718"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03-01", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "5", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "68", "bibliographic_titles": [{"bibliographic_title": "食品総合研究所研究報告"}, {"bibliographic_title": "Report of National Food Research Institute", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A bacterium strain A51-1 producing an inulin fructotransferase (DFAⅠ-producing) [EC 2.4.1.200] in the culture supernatant was isolated. The strain A51-1 was identified as Arthrobacter ureafaciens. The maximum enzyme activity (1.1 U/ml) was obtained with a medium containing 1.5 % yeast extract. The enzyme showed maximum activity at pH 5.5 and 40℃. The enzyme was tolerant up to 70℃. An immobilized enzyme of an inulin fructotransferase (DFAⅠ-producing) was prepared using Chitopearl BCW3510 as a carrier, for the first time. The immobilized enzyme can be used eight times without a significant loss of the activity.\n", "subitem_description_type": "Abstract"}, {"subitem_description": "培養上清中にイヌリンからオリゴ糖DFAⅠを生成する酵素の生産菌 (A51-1) 株を新たに分離した。分類学的な検討の結果、本菌株はArthrobacter ureafaciensと同定された。酵素の生産条件について検討したところ、窒素源として酵母エキスを用いその濃度が1.5%のときにもっとも高い酵素活性が得られた。本酵素はpH5.5, 40℃で最大の高い活性を示し、70℃まで安定であった。キトパールBCW3510を担体とした固定化酵素の調製についても検討した。これはDFAⅠ合成酵素の固定化に関する初めての報告である。本固定化酵素の繰り返し使用を行ったところ、8回の使用後も酵素活性の低下はみられなかった。\n", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24514/00002680", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "農業・食品産業技術総合研究機構"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.24514/00002680 ", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0301-9780", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "原口, 和朋"}, {"creatorName": "ハラグチ, カズトモ", "creatorNameLang": "ja-Kana"}, {"creatorName": "HARAGUCHI, Kazutomo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6718", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006426314", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006426314"}, {"nameIdentifier": "90353961", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90353961"}, {"nameIdentifier": "read0134913", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/read0134913"}]}, {"creatorNames": [{"creatorName": "大坪, 研一"}, {"creatorName": "オオツボ, ケンイチ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "2571", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80353960", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80353960"}, {"nameIdentifier": "keno", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/keno"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-10-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "nfri_report_No69p1-5p.pdf", "filesize": [{"value": "46.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 46000.0, "url": {"label": "nfri_report_No69p1-5p.pdf ", "url": "https://repository.naro.go.jp/record/2797/files/nfri_report_No69p1-5p.pdf"}, "version_id": "a02a1454-5263-4b19-8995-d918d5f4ad80"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Arthrobacter ureafaciensのInulin fructotransferase (DFAⅠ-producing)の生産と固定化", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Arthrobacter ureafaciensのInulin fructotransferase (DFAⅠ-producing)の生産と固定化"}, {"subitem_title": "Production and Immobilization of an Inulin Fructotransferase (DFAⅠ-producing) from Arthrobacter ureafaciens A51-1.", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "12", "path": ["392"], "permalink_uri": "https://doi.org/10.24514/00002680", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-12-20"}, "publish_date": "2019-12-20", "publish_status": "0", "recid": "2797", "relation": {}, "relation_version_is_last": true, "title": ["Arthrobacter ureafaciensのInulin fructotransferase (DFAⅠ-producing)の生産と固定化"], "weko_shared_id": 12}