{"_buckets": {"deposit": "71dc344a-196b-413a-a4eb-ade4effcef06"}, "_deposit": {"created_by": 12, "id": "2787", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "2787"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00002787", "sets": ["391"]}, "author_link": ["2571", "6718"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-03-01", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "24", "bibliographicPageStart": "19", "bibliographicVolumeNumber": "68", "bibliographic_titles": [{"bibliographic_title": "食品総合研究所研究報告"}, {"bibliographic_title": "Report of National Food Research Institute", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A bacterium producing a thermostable inulin fructotransferase (DFAIII-producing) in a culture supernatant was isolated. Through taxonomical studies the strain was identified as Arthrobacter sp. L68-1. The inulin fructotransferase (DFAIII-producing) of the strain showed maximum activity at pH 5.5 and 55℃. The enzyme activity was stable up to 80℃ after 30 min heat treatment. This heat stability was the highest of the inulin fructotransferases (DFAIII-producing) reported to date. The maximum enzyme activity (5.0 U/ ml) was obtained with a medium containing 0.5% Tryptone. An immobilized enzyme was prepared using Chitopearl BCW3510 as a carrier. The immobilized enzyme was able to be used eight times without a significant loss of the enzyme activity.\n", "subitem_description_type": "Abstract"}, {"subitem_description": "耐熱性のDFAIIIオリゴ糖合成酵素を培養上清中に生産する細菌が分離された。分類学的検討の結果本菌株はArthrobacter sp. L68-1と同定された。本菌株の生産するDFAIIIオリゴ糖合成酵素はpH5.5, 55℃において最高の活性を示した。本酵素は30分間の加熱に対して80℃まで安定であった。この耐熱性はこれまでに報告されたDFAIII合成酵素の中で最高であった。酵素の生産条件について検討すると0.5%のトリプトンを含有する培地を用いた場合に最も高い活性が得られた。キトパールBCW3510を担体として固定化酵素を調製した。この固定化酵素を8回繰り返し使用したが、活性の低下は見られなかった。\n", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24514/00002670", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "農業・食品産業技術総合研究機構"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.24514/00002670 ", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0301-9780", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "原口, 和朋"}, {"creatorName": "ハラグチ, カズトモ", "creatorNameLang": "ja-Kana"}, {"creatorName": "HARAGUCHI, Kazutomo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6718", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "9000006426314", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/9000006426314"}, {"nameIdentifier": "90353961", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90353961"}, {"nameIdentifier": "read0134913", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/read0134913"}]}, {"creatorNames": [{"creatorName": "大坪, 研一"}, {"creatorName": "オオツボ, ケンイチ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Ohtsubo, Kenichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2571", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80353960", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80353960"}, {"nameIdentifier": "keno", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/keno"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-10-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "nfri_report_No68p19-24p.pdf", "filesize": [{"value": "67.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 67700.0, "url": {"label": "nfri_report_No68p19-24p.pdf", "url": "https://repository.naro.go.jp/record/2787/files/nfri_report_No68p19-24p.pdf"}, "version_id": "b9887299-14ef-4696-85ea-67b3673dba12"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Arthrobacter sp.L68-1の耐熱性DFAIIIオリゴ糖合成酵素の生産と固定化", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Arthrobacter sp.L68-1の耐熱性DFAIIIオリゴ糖合成酵素の生産と固定化"}, {"subitem_title": "Production and Immobilization of Thermostable Oligosaccharide DFA III Producing Enzyme from Arthrobacter sp. L68-1.", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "12", "path": ["391"], "permalink_uri": "https://doi.org/10.24514/00002670", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-12-20"}, "publish_date": "2019-12-20", "publish_status": "0", "recid": "2787", "relation": {}, "relation_version_is_last": true, "title": ["Arthrobacter sp.L68-1の耐熱性DFAIIIオリゴ糖合成酵素の生産と固定化"], "weko_shared_id": 12}