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Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes
https://repository.naro.go.jp/records/273
https://repository.naro.go.jp/records/27363187247-bc8b-4c3d-8a07-3fc731a3a994
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Animal Science Journal (213.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-03-29 | |||||
| タイトル | ||||||
| タイトル | Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes | |||||
| タイトル | ||||||
| タイトル | Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | additives | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bovine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | in vitro fertilization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | oocyte | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | storage | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | additives | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | bovine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | in vitro fertilization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | oocyte | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | storage | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Tayita, Suttirojpattana
× Tayita, Suttirojpattana× SOMFAI, Tamas× MATOBA, Satoko× Rangsun, Pampai× NAGAI, Takashi× GESHI, Masaya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Our aim was to improve the developmental competence of bovine oocytes during their liquid storage by using additives. In vitro matured oocytes were stored for 20 h at 25°C in HEPES buffered TCM 199 medium (base medium). After storage, in vitro embryo development after in vitro fertilization was compared to those of non‐stored (control) ones. Addition of 10% (v/v) newborn calf serum or 10.27 mmol/L pyruvate alone to the base medium did not improve blastocyst formation rates in stored oocytes; however, their simultaneous addition significantly improved the rate compared with those stored in base medium (P < 0.05). Supplementation of the holding medium with dithiothreitol (DTT) at any concentrations did not improve embryo development from stored oocytes. Although supplementation with cyclosporine A (CsA) significantly reduced apoptosis and membrane damage rates during storage, it did not improve the developmental competence of oocytes. 1,2‐bis(2‐aminophenoxy) ethane N,N,N’,N’‐tetraacetic acid tetrakis‐acetoxymethyl ester and ruthenium red had no effect on oocyte apoptotic rates. Blastocyst formation rates in all stored groups remained significantly lower than that of the control. In conclusion, pyruvate and serum had a synergic effect to moderate the reduction of oocyte quality during storage, whereas mitochondrial membrane pore inhibitor CsA and the antioxidant DTT did not affect their developmental competence. | |||||
| 書誌情報 |
Animal Science Journal en : Animal Science Journal 巻 88, 号 2, p. 231-240, 発行日 2017-02-01 |
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| 出版者 | ||||||
| 出版者 | John Wiley & Sons, Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1740-0929 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1111/asj.12623 | |||||
| 権利 | ||||||
| 権利情報 | © 2016 The Authors. | |||||
| 権利 | ||||||
| 権利情報 | Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science | |||||
| 情報源 | ||||||
| 関連名称 | SC10201707130037 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/asj.12623 | |||||
| 関連名称 | Animal Science Journal | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
