{"_buckets": {"deposit": "d6faa4c9-df1a-4564-bb60-568648a93903"}, "_deposit": {"created_by": 12, "id": "2327", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "2327"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00002327", "sets": ["245"]}, "author_link": ["2157", "457"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-03-22", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "32", "bibliographicPageStart": "23", "bibliographicVolumeNumber": "13", "bibliographic_titles": [{"bibliographic_title": "畜産草地研究所研究報告"}, {"bibliographic_title": "Bulletin of National Institute of Livestock and Grassland Science", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A wild sugarcane clone, Saccharum spontaneum \u0027Glagah Kloet\u0027, is utilized as breeding material for development of high-yielding sugarcane cultivars. In the present study, we established a plant regeneration system for this clone to increase its potential for use in molecular breeding of sugarcane with a transformation system in the future. Although embryogenic callus was not induced with the medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) alone that has been used commonly for callus induction of sugarcane, we obtained embryogenic calli when apical meristems aseptically isolated from shoots were cultured on callus induction medium containing both 0.01 mg L^-1 benzyladenine (BA) and 5 mg L^-1 2,4-D. This finding indicates that BA is positively effective for induction of embryogenic calli of \u0027Glagah Kloet\u0027. The embryogenic calli produced shoots when cultured on medium containing 0.3 mg L^-1 gibberellic acid or on hormone-free medium supplemented with 3 g L^-1 activated charcoal (AC). The morphology of the shoots clearly differed between the regeneration media. The shoots formed on medium containing gibberellic acid were finer and softer, and the leaf color was lighter compared with those formed on the hormone-free medium containing AC, thus more healthy shoots were regenerated.", "subitem_description_type": "Abstract"}, {"subitem_description": "サトウキビ野生種 (SaccharumspontaneumL.) 系統 \"GlagahKloet\" はサトウキビの高収量品種開発のための育種材料としてその利用が期待される。本研究では, 遺伝子組換え技術を軸とした分子育種において本系統の利用を図るため, 本系統のカルス培養からの再分化系確立を試みた。当初, これまでに多くのサトウキビ品種・系統のカルス培養系で利用されてきた 2,4- ジクロロフェノキシ酢酸 (2,4-D) 5mg L^-1 を単独で含むカルス誘導培地に, 幼苗から摘出した茎頂分裂組織を置床したが, 体細胞不定胚形成カルスを得るに至らなかった。しかし, 同培地にさらに低濃度 (0.01mg L^-1) のベンジルアデニン (BA) を添加した培地を使用することでカルス形成が促進され, 体細胞不定胚形成カルスを得ることができた。このことから, 本系統の体細胞不定胚形成カルスの誘導には培地への BA 添加が有効であることが明らかとなった。得られた体細胞不定胚形成カルスを 0.3mg L^-1 ジベレリン (GA) を添加した培地あるいは 3g L^-1 活性炭を加えたホルモンフリー培地に置床することで植物体を再生させることができた。シュートには再生時に使用した培地間で形態的な差が認められ, GA 添加培地上で形成されたシュートは葉の色が薄く, 細長い形態を示し, 軟弱であった。一方, 活性炭を添加したホルモンフリー培地上では健全なシュートが多数形成された。このことから本系統の植物体再生には活性炭を添加したホルモンフリー培地が適していることが示唆された。", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24514/00002226", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "独立行政法人 農業・食品産業技術総合研究機構"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.24514/00002226", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1347-0825", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifier": "", "affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationName": "", "affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "アキリ, 亘", "creatorNameLang": "ja"}, {"creatorName": "アキリ, ワタル", "creatorNameLang": "ja-Kana"}, {"creatorName": "TAKAHASHI, Wataru", "creatorNameLang": "en"}, {"creatorName": "高橋, 亘", "creatorNameLang": "ja"}, {"creatorName": "タカハシ, ワタル", "creatorNameLang": "ja-Kana"}, {"creatorName": "ACKLEY, Wataru", "creatorNameLang": "en"}], "familyNames": [{"familyName": "アキリ", "familyNameLang": "ja"}, {"familyName": "アキリ", "familyNameLang": "ja-Kana"}, {"familyName": "TAKAHASHI", "familyNameLang": "en"}, {"familyName": "高橋", "familyNameLang": "ja"}, {"familyName": "タカハシ", "familyNameLang": "ja-Kana"}, {"familyName": "ACKLEY", "familyNameLang": "en"}], "givenNames": [{"givenName": "亘", "givenNameLang": "ja"}, {"givenName": "ワタル", "givenNameLang": "ja-Kana"}, {"givenName": "Wataru", "givenNameLang": "en"}, {"givenName": "亘", "givenNameLang": "ja"}, {"givenName": "ワタル", "givenNameLang": "ja-Kana"}, {"givenName": "Wataru", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "2157", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70455319", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70455319"}, {"nameIdentifier": "ryegrass", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/ryegrass"}]}, {"creatorNames": [{"creatorName": "高溝, 正"}, {"creatorName": "タカミゾ, タダシ", "creatorNameLang": "ja-Kana"}, {"creatorName": "TAKAMIZO, Tadashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "457", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00355124", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00355124"}, {"nameIdentifier": "takataka", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/takataka"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-03-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ilgs_report_No13p23-32p.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "ilgs_report_No13p23-32p.pdf", "url": "https://repository.naro.go.jp/record/2327/files/ilgs_report_No13p23-32p.pdf"}, "version_id": "8fc18ee6-45b0-4699-bffd-08fe60231087"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "活性炭", "subitem_subject_scheme": "Other"}, {"subitem_subject": "サトウキビ野生種", "subitem_subject_scheme": "Other"}, {"subitem_subject": "植物体再生", "subitem_subject_scheme": "Other"}, {"subitem_subject": "組織培養", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ベンジルアデニン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "activated charcoal", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "benzyladenine", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "plant regeneration", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tissue culture", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "wild sugarcane", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "サトウキビ野生種 (Saccharum spontaneum L.) 系統 \"Glagah Kloet\" のカルスからの植物体再生", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "サトウキビ野生種 (Saccharum spontaneum L.) 系統 \"Glagah Kloet\" のカルスからの植物体再生"}, {"subitem_title": "Plant Regeneration from Embryogenic Calli of the Wild Sugarcane (Saccharum spontaneum L.) Clone \u0027Glagah Kloet\u0027", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "12", "path": ["245"], "permalink_uri": "https://doi.org/10.24514/00002226", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-03-22"}, "publish_date": "2019-03-22", "publish_status": "0", "recid": "2327", "relation": {}, "relation_version_is_last": true, "title": ["サトウキビ野生種 (Saccharum spontaneum L.) 系統 \"Glagah Kloet\" のカルスからの植物体再生"], "weko_shared_id": 12}