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農研機構で実施するカンキツウイルス・ウイロイドのフリー化および検定
https://doi.org/10.24514/00002068
https://doi.org/10.24514/00002068f03a2bc3-3c42-4ef5-8f3b-47045d8327ee
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
nifs_report_No21p53-65p.pdf (903.9 kB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 農研機構で実施するカンキツウイルス・ウイロイドのフリー化および検定 | |||||||||||
| タイトル | ||||||||||||
| タイトル | The Methods for Elimination and Certification of Citrus Virus and Viroid Conducted in NARO, Japan | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | citrus | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | virus | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | viroid | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | elimination | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | certification | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | citrus | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | virus | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | viroid | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | elimination | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | certification | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00002068 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
太田, 智
× 太田, 智
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Prior to distributing scions of new citrus cultivars developed by the Institute of Fruit Tree Science at Japan's National Agriculture and Food Research Organization (NARO), viruses and viroids are eliminated, and the scions are inoculated with a mild Citrus tristeza virus (CTV) strain. NARO has not previously published the method for elimination and certification, and therefore, there have been some inquiries from other organizations. Therefore, we aimed to demonstrate the method for virus and viroid elimination and certification of clean citrus seedlings. We perform shoot tip grafting (Takahara et al. 1986) with some modification to recover virus- and viroid-free plants. Derived seedlings are grafted by approach for growth promotion, according to Takahara et al. (1981). We then test these seedlings for a multitude of viruses and viroids present in Japan, based on RT-PCR. The seedlings must pass the test twice ; in approximately one year, and in one and a half years after approach grafting, they are certified as virus- and viroid-free plants. M16a is used as a mild CTV strain, and the infection is confirmed according to the method of Kano et al. (2006). Virusand viroid-free and M16a-infected trees are kept in isolation structures, and scissors are disinfected at the time of pruning and correcting scions to reduce reinfection. Subsequently, we perform DNA marker typing, create a map of trees, and attach nameplates to avoid mixing up varieties. It would be impossible to certify seedlings as free for every type of virus and viroid. Nevertheless, each research station should choose the best method for the most complete elimination and certification as is possible and should distribute virus- and viroid-free seedlings to prevent multiple infections in orchards. | |||||||||||
| 書誌情報 |
果樹研究所研究報告 en : Bulletin of the National Institute of Fruit Tree Science 巻 21, p. 53-65, 発行日 2016-03-20 |
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| 出版者 | ||||||||||||
| 出版者 | 国立研究開発法人 農業・食品産業技術総合研究機構 果樹茶業研究部門 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1347-3549 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00002068 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
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Cite as
OHTA, Satoshi, 2016, The Methods for Elimination and Certification of Citrus Virus and Viroid Conducted in NARO, Japan: 国立研究開発法人 農業・食品産業技術総合研究機構 果樹茶業研究部門, 53–65 p.
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