{"_buckets": {"deposit": "1e6f015d-5860-418a-9616-15f4d0f801c5"}, "_deposit": {"created_by": 12, "id": "2153", "owners": [12], "pid": {"revision_id": 0, "type": "depid", "value": "2153"}, "status": "published"}, "_oai": {"id": "oai:repository.naro.go.jp:00002153", "sets": ["331"]}, "author_link": ["6229", "6224", "2429", "6089", "1944", "6225", "1633", "5960", "6226", "6227", "1355", "5151", "1661"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-01-31", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "31", "bibliographicPageStart": "25", "bibliographicVolumeNumber": "112", "bibliographic_titles": [{"bibliographic_title": "動物衛生研究所研究報告"}, {"bibliographic_title": "Bulletin of the National Institute of Animal Health", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "An electrochemical sensor for reactive oxygen species like the superoxide anion radical using polymeric iron porphyrin complexes was developed by Yuasa et al. of the Tokyo University of Science. The present experiment was designed to develop a method for rapid measurement of reactive oxygen species in calf blood by employing this technique. The needle-type sensor, including an integrated microelectrode, was dipped into calf blood in a test tube. After xanthine oxidase (O_2^- ・　-generating enzmyme), superoxide dismutase (O_2^- ・ -scavenging enzyme) and opsonized zymosan (phagocyte stimulant) were successively administered in the blood, the current value detected by the sensor in the blood was recorded. The current value increased, decreased and reincreased after the successive administrations of xanthine oxidase, superoxide dismutase and opsonized zymosan, respectively. The sensor was proven to detect the reactive oxygen species of blood. Change in the reactive oxygen species concentration of calf blood following castration was investigated, by dipping the needle-type sensor in the calf blood just after sampling. The reactive oxygen species concentration increased to a maximum at 3 hr, decreasing gradually thereafter following the castration. The fluctuation was almost linked to those of the blood chemiluminescence activity and leukocyte count. Thus, we succeeded in perfoming a rapid measurement of the reactive oxygen species concentration in the peripheral blood of calves.\n", "subitem_description_type": "Abstract"}, {"subitem_description": "東京理科大学の湯浅らによって開発されたスーパーオキシドアニオンラジカルの電気化学的検出法を子牛血液に応用し, 末梢血液中のスーパーオキシドアニオンラジカルなどの活性酸素種濃度を迅速測定する方法を確立するための実験を行った. まず, 当該センサーが子牛血液の活性酸素種濃度を検出しているかどうかを確認するため, 活性酸素種の生成及び消去酵素であるキサンチンオキシダーゼやスーパーオキシドディスムターゼ, 及び貪食細胞刺激剤（貪食細胞からの活性酸素放出刺激剤）であるオプソニン化ザイモザンなどを子牛の血液に投与してセンサーの電流値を調べた. その結果, 当該センサーが間違いなく子牛血液中の活性酸素種を検出していることを確認した. 次に, 当該センサーを用いて子牛の去勢手術に伴う末梢血活性酸素種濃度を7日間にわたって調べたところ, 術後3時間目をピークとする上昇を示し, その後, 緩やかに低下した. この反応は, 末梢血化学発光能や総白血球数の増減にほぼ連動した動きであった. 以上, 子牛血液中の活性酸素種濃度の変化を迅速測定することに成功した. \n", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24514/00002081", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "独立行政法人 農業・生物系特定産業技術研究機構 動物衛生研究所"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.24514/00002081", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1347-2542", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "高橋, 秀之"}, {"creatorName": "タカハシ, ヒデユキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "TAKAHASHI, Hideyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "5151", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "read0004138", "nameIdentifierScheme": "researchmap", "nameIdentifierURI": "http://researchmap.jp/read0004138"}]}, {"creatorNames": [{"creatorName": "宮本, 亨"}, {"creatorName": "ミヤモト, トオル", "creatorNameLang": 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