| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-05-30 |
| タイトル |
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|
タイトル |
A feasibility trial of genomics-based diagnosis detecting insecticide resistance of the diamondback moth |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
insecticide resistance management, diamondback moth, next-generation sequencing, SNP, amplicon sequencing |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
山中, 武彦
Kitabayashi, Satoshi
上樂, 明也
金森, 裕之
桑崎, 誠剛
須藤, 正彬
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
BACKGROUND: Insecticide resistance management has been key for crop protection for over 70 years and is increasingly important because the development of new active ingredients has decreased in recent years. By monitoring the development of resistance in a timely manner, we can effectively prolong insecticide efficacy. Genomic-based diagnosis can reliably predict resistance development if information on resistant mutations against major pesticides is available. Here, we developed a feasibility trial of genomics-based diagnosis of insecticide resistance in diamondback moth (Plutella xylostella) populations in Nagano Prefecture, Japan. Amplicon sequencing analyses using a next-generation sequencer (Illumina MiSeq) for major insecticides, including diamides, pyrethroids, Bacillus thuringiensis (Bt) toxin (Cry1Ac), organophosphates, and spinosyns, were conducted.
RESULTS: Mutations related to the resistance of pyrethroids, organophosphates, and diamides (flubendiamide and chlorantraniliprole) prevailed, while those of a diamide (cyantraniliprole), Bt (Cry1Ac), and spinosyns were scanty, suggesting that they are still effective. The results of the genomics-based diagnosis were generally concordant with the results of bioassays. Resistance development tendencies were generally uniform across Nagano.
CONCLUSION: Insecticide-resistance management campaign can be conducted in Nagano Prefecture with a quick genomic-based diagnosis in early spring while bioassay is the only option for monitoring resistances whose mutations are unavailable. Our study is the first step in the future management of insecticide resistance in all significant pests. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
First published: 03 January 2022 |
|
言語 |
en |
| 書誌情報 |
en : Pest Management Science
巻 78,
号 4,
p. 1573-1581,
発行日 2022-04
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| 出版者 |
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出版者 |
Wiley |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1526-498X |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1002/ps.6776 |
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言語 |
en |
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|
関連名称 |
Publisher's version |
| 権利 |
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|
権利情報 |
© 2022 Society of Chemical Industry. |
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言語 |
en |
| 権利 |
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|
権利情報 |
This is the peer reviewed version of the following article: Yamanaka, T., Kitabayashi, S., Jouraku, A., Kanamori, H., Kuwazaki, S. and Sudo, M. (2022), A feasibility trial of genomics-based diagnosis detecting insecticide resistance of the diamondback moth. Pest Manag Sci, 78: 1573-1581, which has been published in fi nal form at https://doi.org/10.1002/ps.6776. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or bystatutory rights under applicable legislation. Copyright notices must not be removed, obscured or modifi ed. The article must be linked to Wiley’sversion of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third partiesfrom platforms, services and websites other than Wiley Online Library must be prohibited. |
|
言語 |
en |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
postprint_10.1002ps.6776.pdf (2.8 MB)
