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Pretreatment of bovine sperm with dithiobutylamine (DTBA) significantly improves embryo development after ICSI
https://repository.naro.go.jp/records/187
https://repository.naro.go.jp/records/1872997381c-7e04-409c-a807-e892f096f471
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC10201707100004_CCBYNCND_ja.pdf (797.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2018-02-05 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Pretreatment of bovine sperm with dithiobutylamine (DTBA) significantly improves embryo development after ICSI | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Pretreatment of bovine sperm with dithiobutylamine (DTBA) significantly improves embryo development after ICSI | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Bovine | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Dithiobutylamine | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Dithiothreitol | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Intracytoplasmic sperm injection (ICSI) | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Bovine | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Dithiobutylamine | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Dithiothreitol | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Intracytoplasmic sperm injection (ICSI) | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
SUTTIROJPATTANA, Tayita
× SUTTIROJPATTANA, Tayita
× SOMFAI, Tamas
× MATOBA, Satoko
× NAGAI, Takashi
× PARNPAI, Rangsun
× GESHI, Masaya
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | We assessed the effect of pretreating sperm with dithiobutylamine (DTBA) to improve embryo development by intracytoplasmic sperm injection (ICSI) in cows. Acridine Orange staining revealed that when applied at different concentrations (2.5, 5, and 10 mM) and exposure times (5 min, 20 min, 1 h, and 2 h), DTBA reduced disulfide bonds in spermatozoa with the highest efficacy at 5 mM for 5 min. DTBA enhanced the percentage of spermatozoa with free protamine thiol groups compared with untreated spermatozoa (control) (P < 0.05); however, this result did not differ from that of dithiothreitol (DTT) treatment. The percentage of live spermatozoa after DTBA treatment was identical to that in the control, but significantly higher than that after DTT treatment (P < 0.05). After ICSI, DTBA treatment tended to improve male pronuclear formation rate (P = 0.071) compared with non-treated sperm injection. Blastocyst formation rate was significantly improved by DTBA treatment compared with that in DTT, control, and sham injection groups (P < 0.05). Blastocyst quality in terms of cell numbers and ploidy was not different among these groups. In conclusion, DTBA increases the efficacy of blastocyst production by ICSI even if DTT treatment does not work. | |||||||||||||||||
| 書誌情報 |
Journal of Reproduction and Development en : Journal of Reproduction and Development 巻 62, 号 6, p. 577-585, 発行日 2016 |
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| 出版者 | ||||||||||||||||||
| 出版者 | Society for Reproduction and Development | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | 日本繁殖生物学会 | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 1348-4400 | |||||||||||||||||
| DOI | ||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1262/jrd.2016-084 | |||||||||||||||||
| 権利 | ||||||||||||||||||
| 権利情報 | (c)2016 Society for Reproduction and Development | |||||||||||||||||
| 権利 | ||||||||||||||||||
| 権利情報 | (c)2016 日本繁殖生物学会 | |||||||||||||||||
| 情報源 | ||||||||||||||||||
| 関連名称 | SC10201707100004 | |||||||||||||||||
| 関連サイト | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | https://doi.org/10.1262/jrd.2016-084 | |||||||||||||||||
| 関連名称 | Journal of Reproduction and Development | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
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Cite as
SUTTIROJPATTANA, Tayita, SOMFAI, Tamas, MATOBA, Satoko, NAGAI, Takashi, PARNPAI, Rangsun, GESHI, Masaya, 2016, Pretreatment of bovine sperm with dithiobutylamine (DTBA) significantly improves embryo development after ICSI: 日本繁殖生物学会, 577–585 p.
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