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Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs
https://repository.naro.go.jp/records/185
https://repository.naro.go.jp/records/185826afb50-4733-41f6-beec-a099da924713
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC10201707100006_CCBYNCND_en.pdf (802.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2018-02-05 | |||||||||
| タイトル | ||||||||||
| タイトル | Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs | |||||||||
| タイトル | ||||||||||
| タイトル | Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Ca2+ oscillation | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Fertilization | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Intracytoplasmic sperm injection | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Phospholipase C-ζ | |||||||||
| キーワード | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Pig | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Ca2+ oscillation | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Fertilization | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Intracytoplasmic sperm injection | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Phospholipase C-ζ | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Pig | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
NAKAI, Michiko
× NAKAI, Michiko× ITO, Junya× SUZUKI, Shun-ichi× FUCHIMOTO, Daiichiro× SENBON, Shoichiro× SUZUKI, Misae× NOGUCHI, Junko× KANEKO, Hiroyuki
WEKO
998
× Onishi, Akira× KASHIWAZAKI, Naomi× KIKUCHI, Kazuhiro
WEKO
1095
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | In pigs, the efficiency of embryo production after intracytoplasmic sperm injection (ICSI) is still low because of frequent failure of normal fertilization, which involves formation of two polar bodies and two pronuclei. To clarify the reasons for this, we hypothesized that ICSI does not properly trigger sperm-induced fertilization events, especially intracellular Ca2+ signaling, also known as Ca2+ oscillation. We also suspected that the use of in vitro-matured oocytes might negatively affect fertilization events and embryonic development of sperm-injected oocytes. Therefore, we compared the patterns of Ca2+ oscillation, the efficiency of oocyte activation and normal fertilization, and embryo development to the blastocyst stage among in vivo- or in vitro-matured oocytes after ICSI or in vitro fertilization (IVF). Unexpectedly, we found that the pattern of Ca2+ oscillation, such as the frequency and amplitude of Ca2+ rises, in oocytes after ICSI was similar to that in oocytes after IVF, irrespective of the oocyte source. However, half of the oocytes failed to become activated after ICSI and showed no Ca2+ oscillation. Moreover, the embryonic development of normal fertilized oocytes was reduced when in vitro-matured oocytes were used, irrespective of the fertilization method employed. These findings suggest that low embryo production efficiency after ICSI is attributable mainly to poor developmental ability of in vitro-matured oocytes and a lack of Ca2+ oscillation, rather than the pattern of oscillation. | |||||||||
| 書誌情報 |
Journal of Reproduction and Development en : Journal of Reproduction and Development 巻 62, 号 6, p. 615-621, 発行日 2016 |
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| 出版者 | ||||||||||
| 出版者 | Society for Reproduction and Development | |||||||||
| 出版者 | ||||||||||
| 出版者 | 日本繁殖生物学会 | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1348-4400 | |||||||||
| PubMed番号 | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | PMID | |||||||||
| 関連識別子 | 27725347 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1262/jrd.2016-113 | |||||||||
| 権利 | ||||||||||
| 権利情報 | (c)2016 Society for Reproduction and Development | |||||||||
| 権利 | ||||||||||
| 権利情報 | (c)2016 日本繁殖生物学会 | |||||||||
| 情報源 | ||||||||||
| 関連名称 | SC10201707100006 | |||||||||
| 関連サイト | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1262/jrd.2016-113 | |||||||||
| 関連名称 | Journal of Reproduction and Development | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
