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ネギ (Allium fistulosum) の育種における SSR マーカーの応用
https://doi.org/10.24514/00001677
https://doi.org/10.24514/00001677c3d97889-9011-4f9a-bd49-e11dbddf9db2
| 名前 / ファイル | ライセンス | アクション |
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nivts_report_No9p137-188p.pdf (22.2 MB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | ネギ (Allium fistulosum) の育種における SSR マーカーの応用 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Application of SSR Markers for the Breeding of Bunching Onion (Allium fistulosum) | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00001677 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
塚崎, 光
× 塚崎, 光
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Bunching onion (Allium fistulosum L.) is one of the most important vegetables in Japan. However, despite its economic importance, its genetic characteristics are poorly studied. To establish a genetic basis for molecular breeding of bunching onion, I isolated 1796 simple sequence repeat (SSR) clones by large-scale sequencing of SSR-enriched genomic DNA libraries. Of these, 1331 (74.1%) contained (GT)_n repeats (n > 5) and 314 (17.5%) contained (GA)_n repeats. The average numbers of SSRs among the respective clones were 10.5 and 10.4. Five inbred lines contained an average of 3.2 alleles at 100 SSR loci, with a mean polymorphism information content (PIC) of 0.55. These results indicate that bunching onion SSRs arc very rich sources of highly informative genetic markers. I constructed a linkage map of bunching onion (2n = 16) using an F_2 population of 225 plants. The map, covering 2069 cM, consists of 17 linkage groups with 213 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, insertion-deletion (InDel), cleaved amplified polymorphic sequence (CAPS) or derived CAPS (dCAPS) markers. This is the first linkage map based mainly on SSR markers in Allium. Using 103 anchor markers (81 bunching onion SSRs, 11 bulb onion SSRs, and 11 bulb onion non-SSR markers : 1 InDel, 9 CAPSs, 1 dCAPS), I assigned markers to A cepa or A fistulosum chromosomes via the use of several kinds of Allium alien addition lines, and connected 16 of the 17 linkage groups to the 8 basic chromosomes of A cepa. DNA markers can be powerful tools for verifying cultivar identity and genetic homogeneity of F_1 hybrid seed. F_1 cultivars of bunching onion arc becoming prevalent in Japan owing to their high uniformity in agronomic traits. However, bunching onion is an allogamous crop and suffers from severe inbreeding depression when selfed. It is thought that not only open-pollinated cultivars but also parental lines of F_1 hybrids may be bred to hold a degree of genetic heterogeneity. Thus, I evaluated the genetic homogeneity of 8 bunching onion cultivars, including 6 F_1 hybrids, by using 14 SSR markers. The number of alleles detected in the 8 cultivars varied from 3 to 7 among the 14 SSR loci, with PlC values ranging from 0.41 to 0.76. All 8 cultivars showed a low degrees of uniformity at all of these polymorphic loci. Hence, it would seem impossible to determine an appropriate genotypic identity for any existing cultivar of bunching onion. Thus, to improve cultivar identification, I propose an "SSR-tagged breeding" scheme, in which individuals that are homozygous at a few SSR loci are selected out of a foundation seed field. To demonstrate the feasibility of this procedure, I used a bunching onion landrace as starting material for a trial in an allogamous population. Two SSR-tagged populations were produced by selecting double homozygous individuals (population A) and quadruple homozygous individuals (population B) at selected loci. The selected individuals were then open-pollinated within each population. Field growth tests detected no significant differences in any traits tested among the SSR-tagged populations and the control (untagged) populations. ln addition, the SSR-tagged populations were genetically uniform at the selected loci, whereas the other loci still contained high degrees of heterogeneity, suggesting that the SSR-tagged populations can be distinguished from other cultivars by using the selected SSR markers. Therefore, we suggest the "SSR-tagged breeding scheme" as a tool for cultivar identification and for conferring traceability, not only in bunching onion, but also in other allogamous crops that experience severe inbreeding depression. | |||||||||||
| 書誌情報 |
野菜茶業研究所研究報告 en : Bulletin of the National Institute of Vegetable and Tea Science 巻 9, p. 137-188, 発行日 2010-02-26 |
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| 出版者 | ||||||||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 | |||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1346-6984 | |||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00001677 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
