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栽培イチゴにおけるゲノム特異的 DNA マーカーの開発と品種識別技術への応用
https://doi.org/10.24514/00001669
https://doi.org/10.24514/000016695307b352-cbbb-4100-bcaa-937357e0a989
| 名前 / ファイル | ライセンス | アクション |
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nivts_report_No9p7-56p.pdf (8.8 MB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 栽培イチゴにおけるゲノム特異的 DNA マーカーの開発と品種識別技術への応用 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Development of Genome-specific DNA Markers in Strawberry (Fragaria × ananassa Duch.) and Their Use for Cultivar Identification | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00001669 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
國久, 美由紀
× 國久, 美由紀
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Development of genome-specific DNA markers in F. × ananassa Analyses performed in octoploid F. × ananassa (strawberry) by using DNA markers and Mendelian predictions are confounded by the complex and incompletely understood genetic constitution of this species. Therefore, the breeding with genome analysis of F. × ananassa lags far behind other major crops. In this study we tried to develop genome-specific DNA markers which can detect a single genomic locus among multiple homoeologous genomes in F. × ananassa, in order to clarify the genetic constitution of F. × ananassa. In chapter II, section 2, we explain how we developed Cleaved Amplified Polymorphic Sequence (CAPS) markers in the usual way, and we show that they are non-genome-specific and are treated as dominant markers in practical analyses. In section 3, we describe how homoeologous genes amplified by PCR from the octoploid genome were divided into clusters based on their sequences, and primers with cluster-specific sequences were designed to improve the CAPS markers to make them genome-specific. Markers that seemed to be genome-specific were obtained. In section 4, we show how we investigated the inheritance of these improved markers, and we demonstrate that the manner in which they are inherited best suits the model of disomic Mendelian inheritance. Therefore, as expected, the improved markers were proven to be genome-specific. Moreover, the lack of detection of polysomic inheritance in any of the developed markers implied that F. × ananassa is a highly diploidized octoploid (AAA'A'BBB'B'). In section 5, we describe how a search for ancestral species that could have donated a genome to F. × ananassa was performed by detecting genome-specific markers in Fragaria species. At least one genome was thought to be derived from F. vesca. No other ancestral genomes were found among the species tested. Identification of strawberry cultivars using DNA markers The unregulated propagation and distribution of patented domestic strawberry cultivars was first suspected as a serious problem in 2001. Fresh strawberries had been imported from other Asian countries. However, there were strong suspicions that cvs. 'Sachinoka' and 'Tochiotome' might have been mixed with imported strawberries. If these cultivars were imported, the rights of breeders have been infringed, thus inflicting economic damage on domestic producers. In light of these concerns, the development of a practical technique for identifying strawberry cultivars is required. In chapter III, section 2, we show how we tested the utility of the 25 markers we developed in chapter II for cultivar identification and confirmed their ability to distinguish among 117 cultivars, except for mutatorstrains. Validation of the genomo-spocificmarkers as a regulatory tool rests on the reproducibility of the technique as measured by sensitivity and specificity. In section 3, we explain how we performed a collaborative study according to the criteria which the AOAC International has established for qualitative analyses, and we show the high sensitivity and specificity of over 95% for most markers. This is the first report of a technique for cultivar identification with a reproducibility assured through collaborative study. A false positive could possibly result from the accidental generation of a cultivar which has a completely identical genotype as one of the patented cultivars. In section 4, we describe how we calculated the probability of such an event based on the frequency of each genotype detected by independent markers (Ukai, 2004. Theory of cultivar identification). The theoretical accuracy of identification is about 99.9% with 16 independent markers. As a practical test of the methodology, we show the example that imported strawberries from Soyth Korea which were labeled 'Nyoho', were found to be a mixture of cvs. 'Sachinoka' and 'Redpearl', in section 5. | |||||||||||
| 書誌情報 |
野菜茶業研究所研究報告 en : Bulletin of the National Institute of Vegetable and Tea Science 巻 9, p. 7-56, 発行日 2010-02-26 |
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| 出版者 | ||||||||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 | |||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1346-6984 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00001669 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
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Cite as
KUNIHISA, Miyuki, 2010, Development of Genome-specific DNA Markers in Strawberry (Fragaria × ananassa Duch.) and Their Use for Cultivar Identification: 独立行政法人 農業・食品産業技術総合研究機構, 7–56 p.
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