WEKO3
アイテム
日本陸稲およびイネ野生種の縞葉枯病抵抗性に関する遺伝的解析
https://doi.org/10.24514/00001654
https://doi.org/10.24514/000016548bb10bfb-cdca-463b-9065-a8b64255aeb2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
nwarc_report_No7p71-107p.pdf (3.6 MB)
|
|
| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 日本陸稲およびイネ野生種の縞葉枯病抵抗性に関する遺伝的解析 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Genetic analysis for the rice stripe resistance of Japanese upland rice varieties and wild rice species | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 水稲 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 陸稲 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 野生種 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | イネ縞葉枯病 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 準同質遺伝子系統 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | DNAマーカー | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | QTL解析 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 育種 | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00001654 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
前田, 英郎
× 前田, 英郎
WEKO
122
|
|||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Rice stripe disease is one of the most important viral diseases affecting the rice (Oryza sativa L.) production in the temperate regions of East Asia, especially in China, Korea and Japan. The small brown planthopper, Laodelphax striatellus Fallen, transmits the rice stripe virus (RSV), a causal agent of the disease. This disease spreaded in the 1960s in Japan, and an estimated over 600,000 hectares of paddy field was damaged. Screening of RSV-resistant rice varieties was initiated in the early 1960's, and some Indica type rice varieties and Japanese upland rice varieties showed high resistance. An incompletely dominant gene, Stvb-i, controls RSV resistance in Indica type varieties. Resistance in Japanese upland rice varieties was controlled by two complementary genes, Stva and Stvb. Breeding program of RSV-resistant rice was started using Stvb-i gene in Pakistan variety, Modan. Many resistant varieties have been cultivated widely in Japan and these resistant varieties have shown a stable resistance against RSV during the last 40 years. Consequently, almost all the resistant paddy rice varieties cultivated in Japan were expected to harbor this gene. Varieties with a single resistance gene may lose their resistance once the strain of virus changes virulence. For that reason, the other resistant genes should be introduced to the breeding programs of RSV-resistant rice. Some resistant paddy lines were introduced the resistance genes, Stva and Stvb, from Japanese upland rice varieties. However, these lines were also introduced inferior characteristics of grains and eating qualities from the donor parents. In this study, the QTL analysis for rice stripe resistance in the Japanese upland rice Kanto72 (URK72) was performed. Detected QTLs were evaluated and characterized their resistance to RSV using near-isogenic lines (NILs). DNA marker-assisted selection system for rice stripe resistance was examined. Furthermore, novel resistant genes of wild species Oryza rufipogon and O. officinalis were studied. 1. QTL analysis for rice stripe resistance in the Japanese upland rice, Kanto72 To identify the chromosomal segments introduced from upland rice Kanto72 (URK72) into resistant progeny lines, RFLP and SSR marker analysis was performed. Two resistant lines, Chugoku40 and Chugoku41, were investigated their graphical genotypes using 328 RFLP markers and 221 SSR markers distributed on 12 rice chromosomes. In these two resistant lines, chromosomal segments of URK72 were introgressed into chromosomes 2, 6 and 11. RSV resistance genes were thought to be located on these chromosomal regions. A total of 120 F_3 lines derived from a cross between a susceptible variety, Nipponbare, and URK72 was used for QTL analysis for RSV resistance. As a result, two QTLs were detected on the chromosomes 2 and 11. The QTL on chromosome 11 showed large effect for RSV and the LOD peak was detected near a RFLP marker G257. On the other hand, the QTL on chromosome 2 showed small effect for RSV resistance and the LOD peak was detected near a RFLP marker C601. Although the Stva gene was reported to be located on chromosome 6, no QTL for RSV resistance was detected on this chromosome. In order to verify the two QTLs, we selected two informative plants NR8 (heterozygous at the QTL on chromosome 11) and NR23 (heterozygous at the QTL on chromosome 2) from F_2 plants and I used the selfpollinated progenies of NR23 (99 plants) and NR8 (96 plants) for further QTL analyses. In these secondary analyses, one QTL was mapped near the RFLP marker G257 on chromosome 11 and the other QTL was mapped near SSR marker MS-11 on chromosome 2. Since the Stvb gene is allelic with the other RSV resistance gene Stvb-i, which is closely linked to G257, the QTL detected on chromosome 11 was thought to correspond to the Stvb gene. However, the relationship between the QTL on chromosome 2 and the resistance gene Stva was unknown. 2. Characterization of two QTLs controlling resistance to RSV detected in the Japanese upland rice, Kanto 72. The effects of two QTLs were evaluated using near-isogenic lines (NILs). The target QTL regions were introduced from the donor parent Chugoku 40 in the genetic background of Koshihikari. Two NILs possessing a single QTL (QTL-NILs) on chromosomes 2 and 11, respectively, were selected from BC_4F_2 population of the cross between Koshihikari and Chugoku 40 with marker-assisted selection (MAS). Another line, which was a combined QTL-NIL, was developed from the cross between the two QTL-NILs to analyze the interaction of the two QTLs. Investigation of RSV resistance using three NILs revealed that the effects of the two QTLs were clearly differed in the reaction to RSV. The QTL on chromosome 11 had a major effect on reducing the infection rate of RSV. The QTL on chromosome 2 did not affect the infection rate, but made symptoms of diseased plants milder. The combined QTL-NIL showed high resistance to RSV, the infection rate and the symptom types of diseased plants were equivalent to those of URK 72 or Chugoku 40. From these results, RSV resistance identified in URK 72 was thought to be consisted of complementary effect of the two QTLs located on chromosomes 2 and 11. It was reported that the resistance genes, Stva and Stvb, were complementary dominant genes and each gene has no effect against RSV independently. Although the complementary effect of the two QTLs was corresponding to the Stva and Stvb genes, each QTL provided the effects on the suppression of RSV infection and on the suppression of the symptom after infection of RSV. Major agricultural traits of the three QTLNILs were the same to those of Koshihikari, therefore, these QTL-NILs were thought to be useful in rice breeding for RSV resistance as the gene sources. 3. Screening and QTL analysis of the RSV resistance using wild rice species, Oryza rufipogon and O. officinalis. In order to identify novel RSV resistant genes, resistance of wild species was investigated. Firstly, wild species O. rufipogon (A genome) was studied. The QTL analysis was performed using 172 BC_2F_2 lines derived from the crossing between Koshihikari and O. rufipogon (IRGC Acc.104814). A linkage map of 12 chromosomes covering a genetic distance of 1560 cM was constructed using 145 SSR markers. As a result, two QTLs were detected on chromosomes 10 and 11. On chromosome 10, the QTL (LOD=2.6) was found near SSR marker RM333 and this QTL explained about 7.7% of the total phenotypic variation. This QTL was thought to be detected new RSV resistance gene, but it showed small effect against RSV. The QTL on chromosome 11 (LOD=6.1) was detected near SSR marker RM229 and explained about 16.1% of the variation. Secondly, wild species O. officinalis (C genome) was studied. Two resistant lines, WL158R and WL162, were selected from 139 BC_1F_3 lines introgressed chromosomal segment from O. officinalis into cultivated rice. Using 96 F_2 plants/F_3 lines derived from the crossing between Koshihikari (susceptible) and the two resistant lines, QTL analyzes were performed using SSR markers. As results, one major QTL was detected on the long arm of the chromosome 11 in both populations of WL158R and WL162. In this study, the QTLs for RSV resistance were detected on the long arm of chromosome 11 using wild species O. rufipogon and O. officinalis. The detected QTL region of the chromosome 11 was known to be located on the resistant genes Stvb-i and Stvb. The relationships between the QTLs on the chromosome 11 and the two resistant genes were still unknown. Therefore, the relationships should clear through the allelism tests. | |||||||||||
| 書誌情報 |
近畿中国四国農業研究センター研究報告 en : BULLETIN of THE NATIONAL AGRICULTURAL RESEARCH CENTER for WESTERN REGION 巻 7, p. 71-107, 発行日 2008-03-11 |
|||||||||||
| 出版者 | ||||||||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 近畿中国四国農業研究センター | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1347-1244 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00001654 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
