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ホウレンソウにおける植物生育促進根圏細菌利用法の開発
https://doi.org/10.24514/00001620
https://doi.org/10.24514/0000162082722f4d-4d44-4767-9c75-e1fcdc532c05
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
nwarc_report_No6p71-111p.pdf (8.5 MB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||
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| 公開日 | 2019-03-22 | |||||
| タイトル | ||||||
| タイトル | ホウレンソウにおける植物生育促進根圏細菌利用法の開発 | |||||
| タイトル | ||||||
| タイトル | Development of the Application Method for the Plant Growth-Promoting Rhizobacteria | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 蛍光性Pseudomonas | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ホウレンソウ | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PGPR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 有機物 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 画像解析 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 微少熱量計 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 根定着 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 生物発光遺伝子 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.24514/00001620 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
浦嶋, 泰文
× 浦嶋, 泰文 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Rhizobacteria have the potential effect to be useful in agriculture for plant disease control and plant growth promotion. Plant growth-promoting rhizobacteria (PGPR) are soil bacteria that when applied to seeds or roots, are able to colonize plant roots and stimulate plant growth. Strains of fluorescent Pseudomonas, particularly Pseudomonas putida and Pseudomonas fluorescens, belong to a major group of PGPR. Fluorescent Pseudomonas strains (hereafter referred to us "fluorescent Pseudomonas") are able to suppress soil-borne pathogens or promote plant growth. We selected PGPR with the function that promoted the elongation of spinach root using the small-scale sterilized hydroponic culture bioassay system. Several fluorescent Pseudomonas strains which promote growth (the above-ground and root) of the spinach over about 50% was obtained. In the hydroponic culture, inoculated fluorescent Pseudomonas has sufficiently colonized on the spinach root. There was no remarkable growth-promoting effect, in the case of the fluorescent Pseudomonas were inoculated to the spinach in soil culture, while growth promoting effects of the spinach were observed in hydroponic culture. When the bacterized seeds were preserved at 4℃, the decrease of the bacterial population on the seeds were considerably gentle. And, the lowering of the bacterial population on the seeds were held by soaking the seed in the 10g L^-1 methyl cellulose (100 degrees of polymerization). And, the bacterial population on the seeds were maintained high for a long time (180 days), in the case of methyl cellulose were used. Root colonization by a plant growth-promoting Pseudomonas was examined to determine whether gravitational water flow affects the bacterial distribution on spinach roots. The effect of the gravitational water flow by irrigation is important for the spread of colonization of spinach roots by Fluorescent Pseudomonas strains. We considered that the application of organic materials with fluorescent Pseudomonas would promote the PGPR colonization to spinach roots. Therefore, we investigated the behavior of PGPR in the soil treated with or without organic materials. Firstly, motility analysis of Fluorescent Pseudomonas strains were carried out. The motility speed of Fluorescent Pseudomonas strains in this soil solution was analyzed using the image analysis method. The result showed that organic materials that promote the motility of fluorescent Pseudomonas are different for each strain. Secondly, growth of Fluorescent Pseudomonas strains were analyzed using calorimeter. The method using the calorimeter can monitor directly the growth of inoculated bacterial strain in the soil. The results showed that organic matters that promote the growth of Fluorescent Pseudomonas strains are different in each strain. The image analysis system was useful to analyze the motility, and the calorimeter for the growth.Introducing a marker gene into on inoculated strain is an effective way to analyze the actual behavior of the inoculated strain in soil. We introduced a bioluminescence reporter gene of bacterial luciferase into some fluorescent Pseudomonas isolates as a marker to monitor their behavior in the rhizosphere. The transformed strains are bioluminescent and thus provide a rapid and very accurate tool for studies on the population dynamics and spatial distribution of specific bacteria in environmental samples. In conclusion, the expression of the growth-promoting function of fluorescent Pseudomonas in soil culture could be obtained with the application of an appropriate organic material as a carbon source to each of PGPR strains. Though fluorescent Pseudomonas inoculated in soil solely could not colonize the roots due to competition with indigenous microorganisms, the application of organic materials promoted the colonization, resulting in the plant growth-promoting effect of PGPR. Consequently, the method consisting of the use of the appropriate organic materials could be effective for the utilization of fluorescent Pseudomonas in agriculture. | |||||
| 書誌情報 |
近畿中国四国農業研究センター研究報告 en : BULLETIN of THE NATIONAL AGRICULTURAL RESEARCH CENTER for WESTERN REGION 巻 6, p. 71-111, 発行日 2007-03-01 |
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| 出版者 | ||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 近畿中国四国農業研究センター | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1347-1244 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.24514/00001620 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
