エンバク・チオニン遺伝子を導入した組換えイネ系統CT2の隔離圃場における特性調査および安全性評価試験

A transgenic rice line, CT2, was evaluated for its agronomic traits and environmental risk in the isolated paddy field of Hokuriku Research Center, National Agricultural Research Center CT2 was a transgenic rice line with an oat thionin gene which encodes an anti-bacterial protein, thionin. The cloned thionin gene was connected with a constitutive high-expression modified CaMV 35S promoter and introduced to a rice cultivar "Chiyohonami" by Iwai T. et al^(7). The transformants had been evaluated for their resistance to bacterial disease in an isolated green house, and were shown to contain improved resistance against Bulkholderia gulumae and bulkholderia plantarii. A transgenic line, CT2, was selected among the resistant transformants based on its normal growth in the isolated green house for the field performance test of this report. In this evaluation in the isolated paddy fie1d, CT2 showed the obvious resistance against B. gulumae and B. plantarii, as the previous evaluation in the isolated green house. However, the resistance against another bacteria, Acidovorax avenae and Xanthomonos oryzae pv. oryzae, was the same degree as in the original cultivar. In the risk evaluation, there were not any difference between CT2 and the original cultivar in traits, whose alteration could affect the possibility of escape to natural environment and the effect to surrounding ecosystem. Therefore the environmental effect resulted from the cultivation of CT2 would be the same as of the original cultivar. In some agronomic traits, CT2 showed several slight differences to the original cultivar The heading date was 2 days earlier than the original cultivar, the plant height was slightly lower, grains had slightly longer shape, grain weight was slightly larger, seed fertility was slightly lower, and grain yield was 10% lower. Based on the analysis of the observation of these traits, some of these differences were considered to be the results of mutations which were induced during the transformation procedure and the other differences might be caused by the intensive expression of the introduced gene. However, these difference found here might not lead to the difference in the environmental effect of CT2 and the original cultivar, because the differences were small and in every traits CT2 had lesser biological activity Therefore, even with these slight differences, the environmental effect of CT2 would not be different to the original cultivar. To remove the disadvantage of constitutive high-expression of an alien protein, thionin, in rice cell, use of a promoter which confers coleoptile specific high-expression would be idea1, resulting a high level of resistance in the coleoptile whichh is the target organ of the bacteria.

書誌情報

中央農業総合研究センター研究報告
en : Bulletin of the National Agricultural Research Center

巻 2, p. 107-124, 発行日 2003-03-01

出版者

独立行政法人農業技術研究機構中央農業総合研究センター

ISSN

収録物識別子タイプ

ISSN

収録物識別子

1881-6738

DOI

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2023-05-15 16:09:12.313592

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