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園芸植物における日本国内でのウイロイドの発生分布と変異体の感染性
https://doi.org/10.24514/00001435
https://doi.org/10.24514/00001435503f4c00-09c8-4425-af9b-60c6e62743eb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
frc_report_No11p9-48p.pdf (3.1 MB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 園芸植物における日本国内でのウイロイドの発生分布と変異体の感染性 | |||||||||||
| タイトル | ||||||||||||
| 言語 | en | |||||||||||
| タイトル | Distribution of Viroid Variants and Their Infectivity in Horticultural Plants in Japan | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | chrysanthemum | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Chrysanthemum stunt viroid | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Tomato chlorotic dwarf viroid | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | tomato | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | chrysanthemum | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Chrysanthemum stunt viroid | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Tomato chlorotic dwarf viroid | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | tomato | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00001435 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
松下, 陽介
× 松下, 陽介
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Stunting caused by Chrysanthemum stunt viroid (CSVd) is one of the most damaging diseases of cultivated chrysanthemum (Chrysanthemum morifolium), the most important cut flower in Japan. This disease has been reported in many regions of the world. The symptoms are severe stunting of plant height, reduction in flower size, and bleaching of the flower. We assayed for CSVd in cultivated chrysanthemums collected from 10 prefectures in Japan by reverse transcription polymerase chain reaction (RT-PCR) and determined complete nucleotide sequences of CSVd isolates infecting the plants. CSVd was detected in 80 of 89 samples of cultivated chrysanthemum, and samples from all 10 prefectures were infected. Five sequence variants of CSVd were distinguished among 21 isolates based on the differences in the nucleotide sequences. Variant 1 was most frequently detected in samples from 6 prefectures and was assumed to be the predominant CSVd variant occurring in Japan. A viroid disease causing chlorosis of leaves and dwarfism has been found on commercial tomato (Solanum lycopersicum) plants in Hiroshima Prefecture, Japan. Grafting of stems from infected tomatoes onto healthy ones resulted in the healthy plants showing identical symptoms to the infected plants used as a source. Nucleotide sequencing indicated that the causal pathogen was Tomato chlorotic dwarf viroid (TCDVd) sharing 98% nucleotide sequence identity with that of a Canadian isolate reported previously. This description is the first of TCDVd infection of tomato plants in Japan. We investigated the host range and physical properties of TCDVd, with the aim of developing further protective procedures against infection by this viroid. Among the 46 plant species that were inoculated with the viroid, two in the family Compositae and 23 in the family Solanaceae were systemic hosts. The viroids in the crude sap from diseased tomato plants were thermally inactivated by heating at 100℃ for at least 40 min. Viroids also lost their infectivity when diluted in phosphate buffer to at least 10^-6, or after 3 days of incubation at room temperature.CSVd, a non-coding RNA, is known to cause chrysanthemum stunt disease, which affects the yield of flowers. To gain insights into CSVd replication, infection, and the reasons for the spreading of CSVd disease in chrysanthemum plants, we prepared linear CSVd RNA and analyzed its ability to cause disease in chrysanthemum plants. We found that linear CSVd replicated as efficiently as CSVd RNA isolated from infected chrysanthemum plants. Additionally, evaluation of the linear CSVd RNA for its ability to infect other plants revealed that CSVd has a wide host range for its replication. Of, particular concern here that, CSVd isolated from these hosts was found to be infectious to chrysanthemum plants, and thus potentially contributes to the spreading of the disease to chrysanthemum plants. | |||||||||||
| 書誌情報 |
花き研究所研究報告 en : Bulletin of the National Institute of Floricultural Science 巻 11, p. 9-48, 発行日 2011-12-20 |
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| 出版者 | ||||||||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 花き研究所 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1347-2917 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00001435 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
