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キク科植物の花弁におけるカロテノイドに関する研究
https://doi.org/10.24514/00001416
https://doi.org/10.24514/000014168b24e982-54ba-4b92-820b-b27b66da5ab2
| 名前 / ファイル | ライセンス | アクション |
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frc_report_No6p1-54p.pdf (25.3 MB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | キク科植物の花弁におけるカロテノイドに関する研究 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Studies on carotenoids in petals of Compositae plants | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Chrysanthemum morifolium Ramat. | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Calendula officinalis L. | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Compositae | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | petal color | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | carotenoids | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | biosynthetic pathways | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | isomers | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Chrysanthemum morifolium Ramat. | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Calendula officinalis L. | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Compositae | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | petal color | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | carotenoids | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | biosynthetic pathways | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | isomers | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00001416 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
岸本, 早苗
× 岸本, 早苗
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Chrysanthemum (Chrysanthemum morifolium Ramat.) is one of the most important ornamental plants in the world, and its range of petal colors originates mainly from carotenoid and anthocyanin pigments. It is known that the orange color in chrysanthemum petals, which results from the mixture anthocyanins and carotenoids, lacks brightness. In addition, yellow-and white-flowered cultivars are in great demand for funeral ceremonies in Japan, but the quality for cut flowers from yellow-flowered cultivars is generally lower than that of white-flowered cultivars. For this reason, the production of yellow-flowered cultivars has been falling gradually. Because of the importance of controlling flower color, the carotenoid components and the genetic causes that regulate the flower color due to the presence of carotenoids were analyzed in the petals of plants in the Compositae. Twelve chrysanthemum cultivars with petal color ranging from pale yellow to deep red were analyzed by means of high-performance liquid chromatography. No difference was found in their carotenoid composition, but 16 xanthophylls were identified by means of nuclear magnetic resonance (NMR) analysis. Among them, (3S, 5S, 6R, 3'R, 6'R) -5, 6-dihydro-5, 6-dihydroxylutein and five di-Z geometrical isomers of lutein-5, 6-epoxide had never before been identified as natural products. The carotenoid composition, carotenoid content, and expression of genes encoding carotenoid biosynthetic enzymes were also analyzed using the petals and leaves of yellow-and white-flowered chrysanthemums. Most of the carotenoids in yellow petals were β, ε-carotenoids, lutein and its derivatives, reflecting the high expression levels of the gene for lycopene ε-cyclase (LCYE). In contrast, the ratios of β, β-carotenoids to total carotenoids in leaves were higher than those of β, ε-carotenoids to total carotenoids, reflecting the high expression levels of the gene for lycopene β-cyclase (LCYB). A between-cultivar comparison of the expression of the genes encoding carotenoid biosynthetic enzymes in the petals showed no distinct differences between petal colors. The expression of CmCCD1, a highly conserved homologue of the gene for carotenoid cleavage dioxygenase derived from the petals of white-flowered chrysanthemum, was then analyzed. Real-time PCR analysis showed that all white petals that were tested had high levels of expression of CmCCD1, which was not detected in yellow petals. Significant expression of CmCCD1 was strictly limited to the flower petals, therefore we hypothesized that the formation of white petal color resulted from neither down-regulation nor destruction of the carotenoid biosynthesis pathway, but rather to enzymatic cleavage of carotenoids into colorless compounds. Petals of orange-and yellow-flowered cultivars of nine Compositae species were analyzed to determine total anthocyanin content, total carotenoid content, and carotenoid composition in order to clarify the mechanisms responsible for differences in petal color. It became clear that the differences in petal color between orange-and yellow-flowered cultivars were caused by differences in three factors : total anthocyanin content, total carotenoid content, and carotenoid composition. Calendula (Calendula officinalis L.) was one plant that differed in carotenoid composition between orange-and yellow-flowered cultivars. Nineteen carotenoids were identified in extracts from the petals of orange-and yellow-flowered cultivars of calendula by means of NMR analysis, and 10 of them were unique to the orange-flowered cultivars. These 10 were reddish, and it was clear that they were responsible for the orange color of the petals. Among them, (5Z, 9Z)-lycopene, (5Z, 9Z, 5'Z)-lycopene, (5Z, 9Z, 5'Z, 9'Z)-lycopene, (5'Z)-γ-carotene, and (5'Z, 9'Z)-rubixanthin had never before been identified as natural compounds. Since these (5Z)-or (5'Z-carotenoids are unique to orange-flowered cultivars and since yellow-flowered cultivars do not accumulate any 5Z or 5'Z compounds, it appears that C-5 isomerization activity exists only in orange-flowered cultivars. | |||||||||||
| 書誌情報 |
花き研究所研究報告 en : Bulletin of the National Institute of Floricultural Science 巻 6, p. 1-54, 発行日 2007-12-20 |
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| 出版者 | ||||||||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 花き研究所 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1347-2917 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00001416 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
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Cite as
KISHIMOTO, Sanae, 2007, Studies on carotenoids in petals of Compositae plants: 独立行政法人 農業・食品産業技術総合研究機構 花き研究所, 1–54 p.
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