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アカクローバ (Trifolium pratense L.) における DNA マーカーの開発とその育種的利用に関する研究
https://doi.org/10.24514/00001345
https://doi.org/10.24514/00001345b1460317-ea26-40bc-a9c1-2683a44152ec
| 名前 / ファイル | ライセンス | アクション |
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harc_report_No188_01.pdf (2.4 MB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||
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| 公開日 | 2019-03-22 | |||||
| タイトル | ||||||
| タイトル | アカクローバ (Trifolium pratense L.) における DNA マーカーの開発とその育種的利用に関する研究 | |||||
| タイトル | ||||||
| タイトル | Development of DNA markers of red clover (Trifolium pratense L.) and its potential to breeding | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.24514/00001345 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
磯部, 祥子
× 磯部, 祥子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | With the aim of advance the red clover breeding methodology with DNA markers, DNA markers of redclover (Trifolium pratense L.) were developed and evaluated its potential to breeding. 1. A genetic linkage map of red clover was constructed using RFLP markers from cDNA probes of a backcrossed mapping population. The map contains 157 RFLP markers and one morphological marker on 7 linkage groups. The total map distance was 535.7cM and the average distance between two markers was 3.4cM. This is the first report of construction of a linkage map of Trifolium species. 2. A high-density genetic linkage map was established with gene-associated microsatellite markers. A total of 7159 primer pairs were designed to amplify simple sequence repeats (SSRs) identified in four different types of libraries. Based on sequence similarity, 82% of the SSRs were likely to be associated with genes. The 1305 selected microsatellite markers as well as 167 previously developed RFLP markers were subjected to linkage analysis. A total of 1434 loci detected by 1399 markers were successfully mapped onto seven linkage groups totaling 868.7 cM in length. Each genetic linkage group was linked to a corresponding chromosome by FISH analysis using seven microsatellite markers specific to each of the linkage groups as probes. Macrosynteny at the segmental level was observed between the genomes of red clover and two model legumes, Lotus japonicus and Medicago truncatula, strongly suggesting that the genome information for the model legumes is transferable to red clover for genetic investigations and experimental breeding. 3. The transferability RFLP and microsatellite markers to other red clover germplasms were investigated. The mean values of allele numbers per RFLP and microsatellite marker were 3.3 and 6.5, respectively. A linkage map was reconstructed with 90 cDNA probes of RFLP markers and 177 primer pairs of microsatellite markers by 272×WF1680 mapping population, and 301 loci were mapped onto the linkage map totaling 747.5 cM. All primer pairs of microsatellite markers were mapped onto the same linkage groups of HR×130 mapping population, while 16% of cDNA probes of RFLP markers were mapped onto different linkage groups. The microsatellite markers were considered to have high transferability and universality to breeding materials. 4. QTL analysis for winter hardiness and Sclerotinia trifoliorum resistance was performed with the RFLP and microsatellite linkage maps. The phenotypic traits of the mapping populations, 272×WF1680 and HR×R130, were estimated at National Agricultural Research Center for Hokkaido Region in Sapporo and All-Russian Williams Fodder Crop Research Institute in Moscow region, Russia. Most phenotypic traits showed no significant correlation. However, many of them detected significant QTLs on the same locus of the maps, especially on LG6. We considered that the QTLs on LG6 were a potent region in which mapped candidate selection markers are related to winter hardiness. In conclusion, we developed RFLP and microsatellite markers and constructed two linkage maps of red clover. The results suggest that the information has a high potential for improving red clover breeding methodology. y | |||||
| 書誌情報 |
北海道農業研究センター研究報告 en : Research BUlletin of the NARO Hokkaido Agricultural Research Center 巻 188, p. 1-53, 発行日 2008-03-01 |
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| 出版者 | ||||||
| 出版者 | 独立行政法人 農業・食品産業技術総合研究機構 北海道農業研究センター | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1347-8117 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.24514/00001345 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
