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サテライト RNA遺伝子の導入によるキュウリモザイクウイルス抵抗性トマトに関する研究 : 第1報 抵抗性トマト系統の作出
https://doi.org/10.24514/00001310
https://doi.org/10.24514/0000131079f2708f-612e-4769-8a7f-b07838ff9a1e
| 名前 / ファイル | ライセンス | アクション |
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harc_report_No177p37-53p.pdf (207.6 kB)
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| Item type | 紀要論文01 / Departmental Bulletin Original Article(1) | |||||||||||
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| 公開日 | 2019-03-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | サテライト RNA遺伝子の導入によるキュウリモザイクウイルス抵抗性トマトに関する研究 : 第1報 抵抗性トマト系統の作出 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Virus resistance in transgenic tomato expressing satellite RNA of Cucumber mosaic virus 1 : Genetical aspects and virus resistance | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24514/00001310 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
河辺, 邦正
× 河辺, 邦正× 岩崎, 眞人× 早野-斎藤, 由里子
WEKO
861
× 本田, 要八郎× 吉田, 幸二× 眞岡, 哲夫 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The complementary DNA of a symptom suppressor strain of Cucumber mosaic virus (CMV) satellite RNA were transformed into a Japanese cultivar of tomato, 'Shugyoku', to obtain CMV resistant tomato. The resistance of transgenic plants was examined in second generation of self-crossed lines which have satellite cDNA at a single locus in homozygous. A highly resistant line, 'No.4-7' shows only very mild symptom when inoculated with virulent CMV isolates with no satellite RNA. When CMV-Y containing satellite RNA of yellow mosaic type was inoculated, the 'No.4-7' shows symptoms in early phase of infection. However, two or three weeks after inoculation, the transgenic line recovers the growth and shows only mild mosaic symptom. The 'No.4-7' shows no resistance against CMV with necrogenic satellite RNA. The virulent CMV solates become attenuated through multiplication in the transgenic tomato. This indicates that the inoculated CMV acquires the satellite RNA in the transgenic tomato. After the acquisition by CMV, the satellite RNA of transgenic tomato is aphid-transmissible to non-transgenic tomato or tobacco with CMV. The transgenic tomato is consideredto produce novel satellite RNA with the samenatures as existing viral RNA in the field. The biosafety assessment of transgenic tomatoline, 'No.4-7' will be undertaken to develop commercial CMV resistant line. | |||||||||||
| 書誌情報 |
北海道農業研究センター研究報告 en : Research BUlletin of the NARO Hokkaido Agricultural Research Center 巻 177, p. 37-53, 発行日 2003-12-01 |
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| 出版者 | ||||||||||||
| 出版者 | 独立行政法人 農業技術研究機構 北海道農業研究センター | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1347-8117 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.24514/00001310 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
