| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-03-29 |
| タイトル |
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|
タイトル |
Effects of polyethylene glycol and a synthetic ice blocker during vitrification of immature porcine oocytes on survival and subsequent embryo development. |
| タイトル |
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タイトル |
Effects of polyethylene glycol and a synthetic ice blocker during vitrification of immature porcine oocytes on survival and subsequent embryo development. |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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主題Scheme |
Other |
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主題 |
Immature oocyte |
| キーワード |
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主題Scheme |
Other |
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主題 |
Pig |
| キーワード |
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主題Scheme |
Other |
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主題 |
Polyethylene glycol |
| キーワード |
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主題Scheme |
Other |
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主題 |
Synthetic ice blocker |
| キーワード |
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主題Scheme |
Other |
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主題 |
Vitrification |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Immature oocyte |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pig |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
Polyethylene glycol |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
Synthetic ice blocker |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
Vitrification |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
da Silva Santos, Elisa Caroline
ソムファイ, タマス
Appeltant, Ruth
ダン・グェン, タィン・クァン
野口, 純子
菊地, 和弘
KANEKO, Hiroyuki
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We evaluated the effects of polyethylene glycol (PEG) and Supercool X‐1000 (SC) as supplements during the vitrification of immature cumulus‐enclosed porcine oocytes in a solution based on 17.5% ethylene glycol + 17.5% propylene glycol. After warming, the oocytes were subjected to in vitro maturation, fertilization and embryo culture. In Experiment 1, equilibration and vitrification solutions were supplemented with or without 2% (w/v) PEG (PEG+ and PEG‐, respectively). The survival rate, cleavage and blastocyst development were similar between PEG+ and PEG‐ groups; however, all values were lower than those in the non‐vitrified control. In Experiment 2, vitrification solution was supplemented with or without 1% (v/v) SC (SC+ and SC‐, respectively). The percentages of survival and blastocyst development were similar between SC+ and SC‐ groups but lower than those in the non‐vitrified control. The percentage of cleavage in the SC‐ group was significantly lower than the control and the SC+ groups, which were in turn similar to one another. In both experiments, the cell numbers in blastocysts were not significantly different among the non‐vitrified and vitrified groups. In conclusion, PEG did not improve oocyte survival and embryo development, whereas SC improved the ability of surviving oocytes to cleave but not to develop into blastocysts. |
| 書誌情報 |
Animal Science Journal
en : Animal Science Journal
巻 88,
号 8,
p. 1042-1048,
発行日 2017-08-18
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| 出版者 |
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出版者 |
Japanese Society of Animal Science |
| 出版者 |
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出版者 |
John Wiley & Sons, Inc. |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1740-0929 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/asj.12730 |
| 権利 |
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権利情報 |
This is the peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/abs/10.1111/asj.12730], which has been published in final form at [https://doi.org/10.1111/asj.12730]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| 権利 |
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権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご利用ください。 |
| 権利 |
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権利情報 |
This is not the published version. Please cite only the published version. |
| 情報源 |
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関連名称 |
SC10201707060028 |
| 関連サイト |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/asj.12730 |
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関連名称 |
Animal Science Journal |
| 著者版フラグ |
|
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
SC10201707060028_Manuscript_Santos_Revised_finalforPDF.pdf (346.6 kB)
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