| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-01-18 |
| タイトル |
|
|
タイトル |
Construction and characterization of a full-length infectious cDNA clone of foot-and-mouth disease virus strain O/JPN/2010 isolated in Japan in 2010 |
| タイトル |
|
|
タイトル |
Construction and characterization of a full-length infectious cDNA clone of foot-and-mouth disease virus strain O/JPN/2010 isolated in Japan in 2010 |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Foot-and-mouth disease virus |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Full-length cDNA |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Pathogenicity |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
O/JPN/2010 strain |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Foot-and-mouth disease virus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Full-length cDNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pathogenicity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
O/JPN/2010 strain |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
西, 達也
小野里, 洋行
大橋, 誠一
深井, 克彦
山田, 学
森岡, 一樹
菅野, 徹
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A full-length infectious cDNA clone of the genome of a foot-and-mouth disease virus isolated from the 2010 epidemic in Japan was constructed and designated pSVL-f02. Transfection of Cos-7 or IBRS-2 cells with this clone allowed the recovery of infectious virus. The recovered virus had the same in vitro characterization as the parental virus with regard to antigenicity in neutralization and indirect immunofluorescence tests, plaque size and one-step growth. Pigs were experimentally infected with the parental virus or the recombinant virus recovered from pSVL-f02 transfected cells. There were no significant differences in clinical signs or antibody responses between the two groups, and virus isolation and viral RNA detection from clinical samples were similar. Virus recovered from transfected cells therefore retained the in vitro characteristics and the in vivo pathogenicity of their parental strain. This cDNA clone should be a valuable tool to analyze determinants of pathogenicity and mechanisms of virus replication, and to develop genetically engineered vaccines against foot-and-mouth disease virus. |
| 書誌情報 |
Research in Veterinary Science
en : Research in Veterinary Science
巻 106,
p. 165-169,
発行日 2016-06
|
| 出版者 |
|
|
出版者 |
Elsevier |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0034-5288 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
27234555 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.rvsc.2016.03.017 |
| 権利 |
|
|
権利情報 |
(C) 2016 Elsevier Ltd. |
| 権利 |
|
|
権利情報 |
Green open Access |
| 権利 |
|
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご利用ください。 |
| 権利 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 情報源 |
|
|
|
関連名称 |
SC30201707130014 |
| 関連サイト |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.rvsc.2016.03.017 |
|
|
関連名称 |
Research in Veterinary Science |
| 他の資源との関係 |
|
|
|
関連名称 |
author manuscript |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
SC30201707130014 _postprint.pdf (2.4 MB)
.png)
