A new two-rowed barley cultivar "Koharu Nijo" was bred by the pedigree breeding method at the National Agricultural Research Center for the Tohoku Region in 2008. It was selected from a cross between F_1 ("Nishino Gold" / "Miyukioomugi") and "Kyushu Nijo 11 (Miharu Gold)". "Koharu Nijo" is a remarkably improved high quality cultivar for malting and milling with awns, hulled barley, spring habit is degree II. "Koharu Nijo" is classified as having moderate cold tolerance and slightly weak snow mold tolerance, but its cold and snow mold tolerance are higher than those of other two-rowed barley cultivars. "Koharu Nijo" is classified as a slightly late maturity barley in the Tohoku region with a slightly long culm length. It has a higher spike number, lower yielding ability, and higher thousand-grain weight than the standard six-rowed barley cultivar "Minorimugi" in the Tohoku and Hokuriku regions. "Koharu Nijo" has moderate pre-harvest sprouting resistance and moderate scab resistance. Its barley yellow mosaic resistance and powdery mildew resistance are intermediate, and its rust resistance is low. The malt extract and diastatic power per total nitrogen of "Koharu Nijo" is higher than that of "Minorimugi". This cultivar has been suggested for local production for use by a microbrewery. The whiteness of the pearled grain is the same as that of "Minorimugi." "Koharu nijo" is considered to be adapted to low lands in the Tohoku and Hokuriku regions in Japan.