This paper describes a field study jointly conducted by Myanmar and Japan in February 2019 to document the plant genetic resources (PGRs) of Chin State, Myanmar. The study was a part of PGRAsia, an international joint research project between the Japanese research institutes and gene banks of Asian countries, consigned by the Ministry of Agriculture, Forestry and Fisheries to the National Agriculture and Food Research Organization (NARO) of Japan. It was implemented under a Memorandum of Understanding between NARO and the Department of Agricultural Research (DAR) of Myanmar, which was originally co-signed in 2013 and extended with an addendum in 2018. Previous surveys have indicated that the diversity of the traditional crops of inland Chin State are on the verge of lost, and in the present survey, we focused on plant resources of the mid-south areas of Chin State, including Madupi District. Based on the findings of a previous short survey conducted by Ohm Mar Saw et al. (2019) in 2018, we focused on areas surrounding the Madupi Township during the agricultural off-season to collect PGRs and associated information. We collected PGRs mainly from the Mindat Township (Mindat District), Madupi and Retzua Townships (Madupi District), and Hakha Township (Hakha District) and a few PGRs from the Pale Township (Yinmabin District, Sagaing Region) and Pyinmana Township (Naypyidaw Union Territory). A total of 178 plant samples were collected which belonged to the following families: Amaranthaceae (five samples), Apiaceae (two), Araceae (four), Brassicaceae (11), Cucurbitaceae (26), Dioscoreaceae (two), Fabaceae (43), Lamiaceae (13), Malvaceae (11), Pedaliaceae (two), Poaceae (42), Rubiaceae (one), Solanaceae (13), and Zingiberaceae (three). The collected PGRs were divided into two subsets – one to be investigated and conserved at the DAR Seed Bank of Myanmar and the other at the NARO Genetic Resources Center of Japan. Interviews with locals revealed that the vernacular names of selected crop plants were similar within a given Township in Chin State but often differed between the Townships. A 2005 field study on wild rice varieties of Chin State reported the occurrence of foxtail millet, and we anticipated finding this millet and were able to collect eight seed samples. However, none of these was viable in a germination test, and this indicates an ongoing genetic erosion of plant genetic resources in the study area.