{"created":"2023-05-15T13:41:36.681730+00:00","id":6775,"links":{},"metadata":{"_buckets":{"deposit":"8fd6c2b1-df20-4ba4-ae4b-7b43299bad3b"},"_deposit":{"created_by":11,"id":"6775","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"6775"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00006775","sets":["1706970004180:1:420:554","87:653:40"]},"author_link":["7385","943","399","898","418"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"80","bibliographicPageStart":"73","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"農研機構研究報告"},{"bibliographic_title":"Journal of the NARO Research and Development","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In Japan, cultivars ‘Kober 5BB’ (Vitis berlandieri × Vitis riparia), ‘Riparia Gloire’ (V. riparia) ‘101-14’ (V. riparia × Vitis rupestris) are used as rootstocks for table or wine grapes. As newly emerging viruses were detected from some mother rootstocks, elimination of viruses from the three cultivars was performed using thermotherapy and meristem culture. Among sixteen to eighteen clones obtained, we selected six clones each in the first screening by reverse-transcription polymerase chain reaction (RT-PCR). Mixed RNA extracts from the six were analyzed by next-generation sequencing. No viruses were detected from all the clones, although some of them tested positive for viroids. Consequently, no viruses and viroids were detected from two to six clones of all the cultivars. The clones will be utilized as rootstocks for virus-free vines in Japan.\n ","subitem_description_type":"Abstract"},{"subitem_description":"国内における生食用ブドウあるいは醸造用ブドウ栽培では, 「テレキ5BB」(Vitis berlandieri × Vitis riparia),「グロワール」(V. riparia), 「101-14」(V. riparia × Vitis rupestris) などが代表的な台木品種として使用される. 一部の台木母樹に, 新種ウイルスの感染事例が認められたことから, 熱処理と茎頂培養の併用による無毒化個体の作出を行った. 得られた16～18個体について, 逆転写ポリメラーゼ連鎖反応（RT-PCR）により一次選抜を行った. 選抜した6個体ずつを混合して, 現時点で最も信頼性の高い新たな手法である次世代シーケンス解析を用いたウイルス診断を行った. その結果, 「テレキ5BB」の一部の個体でウイロイドの陽性が認められた他は, 全ての供試個体においてウイルス感染は認められなかった. それぞれの2～6個体からはいずれのウイルス・ウイロイドも検出されず, 無毒化台木母樹としての利用が期待できる.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00006635","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構"},{"subitem_publisher":"National Agriculture and Food Research Organization, Japan (NARO)"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.34503/naroj.2021.7_73","subitem_relation_type_select":"DOI"}}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"本文はJ-Stageよりご利用いただけます。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This article full-text PDF is available at J-Stage."}]}]},"item_10002_relation_17":{"attribute_name":"全文リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.34503/naroj.2021.7_73"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.34503/naroj.2021.7_73","subitem_relation_type_select":"DOI"}}]},"item_10002_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"2022.2 J-Stageへ移管しました"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"We changed the journal platform to J-Stage.　2022.2 -"}]}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2434-9909","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"千秋, 祐也"},{"creatorName":"チアキ, ユウヤ","creatorNameLang":"ja-Kana"},{"creatorName":"CHIAKI, Yuya","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中島, 育子"},{"creatorName":"ナカジマ, イクコ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAJIMA, Ikuko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"土師, 岳"},{"creatorName":"ハジ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"HAJI, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"佐藤, 明彦"},{"creatorName":"サトウ, アキヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"SATO, Akihiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"伊藤, 隆男"},{"creatorName":"イトウ, タカオ","creatorNameLang":"ja-Kana"},{"creatorName":"ITO, Takao","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-13"}],"displaytype":"detail","filename":"10.24514 00006635_DOI_moved.pdf","filesize":[{"value":"298.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"10.24514 00006635_DOI_moved","url":"https://repository.naro.go.jp/record/6775/files/10.24514 00006635_DOI_moved.pdf"},"version_id":"261217ef-d79a-468a-b15e-2c4c7d66cd1b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"次世代シーケンス解析","subitem_subject_scheme":"Other"},{"subitem_subject":"台木","subitem_subject_scheme":"Other"},{"subitem_subject":"ブドウ","subitem_subject_scheme":"Other"},{"subitem_subject":"無毒化","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"certification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"elimination","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"grapevine rootstock","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"next-generation sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ブドウ台木3品種の無毒化と, RT-PCRおよび次世代シーケンス解析を利用したウイルス・ウイロイド検定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ブドウ台木3品種の無毒化と, RT-PCRおよび次世代シーケンス解析を利用したウイルス・ウイロイド検定"},{"subitem_title":"Elimination of viruses from three grapevine rootstock cultivars and certification using RT-PCR and next-generation sequencing","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["40","554"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-07-30"},"publish_date":"2021-07-30","publish_status":"0","recid":"6775","relation_version_is_last":true,"title":["ブドウ台木3品種の無毒化と, RT-PCRおよび次世代シーケンス解析を利用したウイルス・ウイロイド検定"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T16:20:25.889339+00:00"}